PUBLICATION
Developmental single-cell atlas of coronary growth and cardiomyocyte interaction in zebrafish
- Authors
- Rouf, M.A., Kürekçi, G.K., Zhang, S., Vanier, S.L., Kamel, S.M., Lee, A.N., Yang, R.B., Lai, S.L., Marín-Juez, R.
- ID
- ZDB-PUB-260114-5
- Date
- 2026
- Source
- Development (Cambridge, England) : (Journal)
- Registered Authors
- Kamel, Sarah, Lai, Shih-Lei (Ben), Marín-Juez, Rubén, Rouf, Muhammad Abdul, Yang, Ruey-Bing (Ray)
- Keywords
- Cardiomyocytes, Cardiovascular, Coronary vasculature, Development, Vegfa, Zebrafish
- Datasets
- GEO:GSE298874
- MeSH Terms
-
- Animals
- Cell Communication
- Coronary Vessels*/cytology
- Coronary Vessels*/embryology
- Coronary Vessels*/growth & development
- Coronary Vessels*/metabolism
- Endothelial Cells/metabolism
- Gene Expression Regulation, Developmental
- Heart/embryology
- Myocytes, Cardiac*/cytology
- Myocytes, Cardiac*/metabolism
- Signal Transduction
- Single-Cell Analysis/methods
- Vascular Endothelial Growth Factor A/genetics
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 41531417 Full text @ Development
Citation
Rouf, M.A., Kürekçi, G.K., Zhang, S., Vanier, S.L., Kamel, S.M., Lee, A.N., Yang, R.B., Lai, S.L., Marín-Juez, R. (2026) Developmental single-cell atlas of coronary growth and cardiomyocyte interaction in zebrafish. Development (Cambridge, England). :.
Abstract
Cardiac morphogenesis requires the intricate coordination of different cell types and molecular cues. Coronary vessel formation is essential for heart development, yet how coronaries grow and contribute to ventricular wall formation remains poorly understood. Here, we combine high-resolution imaging and new genetic tools to systematically analyze coronary development in zebrafish at each millimeter increment from 7 to 30 mm of body length and identify cellular and molecular milestones defining four distinct coronary network developmental stages. Our data show how coronary vessels expand, pattern, specify and act as vascular scaffolds to guide cardiomyocyte growth throughout development. Manipulating coronary network growth via Vegfa signaling perturbation through gain- and loss-of-function approaches disrupts coronary-cardiomyocyte interactions and cardiomyocyte expansion. To gain further resolution into these processes, we build a single-cell RNA sequencing atlas by profiling 37,554 ventricular cells across the different developmental stages and identified new coronary markers, dynamic cellular interactions, and stage-specific endothelial-cardiomyocyte crosstalk. Overall, we present the first comprehensive roadmap of coronary vessel formation and coordinated cellular and molecular interactions with the developing cardiac muscle in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping