PUBLICATION
Anwulignan mitigates UVB-induced skin photodamage by dual activation of the Nrf2/PINK1/Parkin and Nrf2/SLC7A11/GPX4 signaling pathways
- Authors
- Jiang, Y., Wang, D., Lin, X., Yuan, Z., Xing, X., Gao, Q., Zhang, T., Jing, S., Li, H.
- ID
- ZDB-PUB-260108-8
- Date
- 2025
- Source
- Phytomedicine : international journal of phytotherapy and phytopharmacology 150: 157692 (Journal)
- Registered Authors
- Keywords
- Antioxidant, Anwulignan, Ferroptosis, Mitophagy, Skin photoaging
- MeSH Terms
-
- Animals
- Glutathione Peroxidase/metabolism
- Male
- Mice
- Molecular Docking Simulation
- NF-E2-Related Factor 2/metabolism
- Oxidative Stress/drug effects
- Protein Kinases/metabolism
- Signal Transduction/drug effects
- Skin/drug effects
- Skin/radiation effects
- Skin Aging*/drug effects
- Skin Aging*/radiation effects
- Ubiquitin-Protein Ligases/metabolism
- Ultraviolet Rays/adverse effects
- Zebrafish
- PubMed
- 41499935 Full text @ Phytomedicine
Citation
Jiang, Y., Wang, D., Lin, X., Yuan, Z., Xing, X., Gao, Q., Zhang, T., Jing, S., Li, H. (2025) Anwulignan mitigates UVB-induced skin photodamage by dual activation of the Nrf2/PINK1/Parkin and Nrf2/SLC7A11/GPX4 signaling pathways. Phytomedicine : international journal of phytotherapy and phytopharmacology. 150:157692.
Abstract
Background Skin photoaging (SP) is a major contributor to skin aging. Anwulignan (AN) has been confirmed to possess diverse biological activities. This study investigated the protective effect of AN against SP and explored its underlying mechanisms.
Methods A UVB-induced SP model in mice was established to evaluate skin erythema index (EI), transepidermal water loss (TEWL), and skin hydration. Histopathological assessments were performed using hematoxylin and eosin (HE), Masson, and Sirius red staining. Transmission electron microscopy examined the morphology and quantity of mitochondria in epidermal cells. ELISA measured levels of hydroxyproline (HYP), hyaluronic acid (HA), oxidative stress markers, and inflammation indicators. Molecular docking predicted the potential targets of AN on UVB-induced SP. Western blot and immunofluorescence staining assessed the expression of proteins related to oxidation, inflammation, aging, ferroptosis, and autophagy. Zebrafish experiments further validated the effects.
Results AN significantly alleviated SP, evidenced by increased levels of HA and HYP, suppressed pro-inflammatory cytokine expression, reduced reactive oxygen species (ROS), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and malondialdehyde (MDA), while enhancing activities of total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). It also regulated the expression of proteins involved in the Nrf2/PINK1/Parkin mitophagy axis and the Nrf2/SLC7A11/GPX4 ferroptosis regulatory axis. Significant differences were observed among groups in tail fin area, β-galactosidase staining fluorescence intensity, yolk sac fluorescence, and mitochondrial fluorescence in muscle.
Conclusion AN may exert protective effects against SP by targeting Nrf2 and subsequently activating both the Nrf2/PINK1/Parkin mitophagy axis and the Nrf2/SLC7A11/GPX4 ferroptosis regulatory axis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping