PUBLICATION
Microglia response and function in a chronic model of photoreceptor damage
- Authors
- Raghavan, D., Jeakle, O., Berry, Y., Victor, M., Thummel, R.
- ID
- ZDB-PUB-260101-8
- Date
- 2025
- Source
- Frontiers in cell and developmental biology 13: 16992711699271 (Journal)
- Registered Authors
- Thummel, Ryan
- Keywords
- Muller glia, degeneration, microglia, photoreceptor, zebrafish
- MeSH Terms
- none
- PubMed
- 41476611 Full text @ Front Cell Dev Biol
Citation
Raghavan, D., Jeakle, O., Berry, Y., Victor, M., Thummel, R. (2025) Microglia response and function in a chronic model of photoreceptor damage. Frontiers in cell and developmental biology. 13:16992711699271.
Abstract
Background Retinal neurodegenerative diseases, including diabetic retinopathy and age-related macular degeneration, are characterized by the slow, chronic degeneration of photoreceptors. We previously used a chronic low light (CLL) exposure to model slow photoreceptor degeneration in adult zebrafish. Here, we investigate transcriptional, morphological, and functional responses of microglia in the CLL model.
Methods Microglia-specific gene expression analysis was mined from our previously reported 3' RNA-seq data performed at 8 time points during 28 days of CLL exposure. Morphological changes were performed on retinas collected at various time points using immunohistochemistry. Microglial inhibition was accomplished pharmacologically with dexamethasone and genetically using the irf8-/- mutant fish. Finally, we returned the CLL-treated fish to normal light/dark conditions to test whether photoreceptors could recover in the context of chronic stress.
Results CLL induced dynamic, time-dependent upregulation of microglia-specific genes consistent with pro-inflammatory and pro-resolving function. Dexamethasone treatment reduced microglial numbers and exacerbated rod and cone outer segment damage, whereas irf8-/- mutants exhibited partial protection against photoreceptor damage. Notably, despite prolonged stress and damage during the CLL exposure, photoreceptor outer segments returned to near-baseline morphology after 28 days of normal light/dark recovery conditions.
Discussion Overall, these findings suggest that microglial function in chronic retinal injury is context-dependent as pharmacological and genetic methods of inhibition produced contrasting outcomes depending upon microglial polarization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping