PUBLICATION
Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures
- Authors
- Kumar, A., Singh, D.
- ID
- ZDB-PUB-251207-3
- Date
- 2025
- Source
- Zebrafish 22: 223229223-229 (Journal)
- Registered Authors
- Singh, Damanpreet
- Keywords
- Danio rerio, c-fos, clonus-like seizure, epilepsy, immediate early genes, pentylenetetrazole (PTZ)
- MeSH Terms
-
- Larva/drug effects
- Larva/genetics
- Larva/metabolism
- Zebrafish*/genetics
- Zebrafish*/growth & development
- Zebrafish*/metabolism
- Proto-Oncogene Proteins c-fos*/genetics
- Proto-Oncogene Proteins c-fos*/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Animals
- Seizures*/chemically induced
- Seizures*/genetics
- Seizures*/metabolism
- Time Factors
- Pentylenetetrazole*/toxicity
- Disease Models, Animal
- PubMed
- 41351512 Full text @ Zebrafish
Citation
Kumar, A., Singh, D. (2025) Temporal Dynamics of c-Fos Expression in a Zebrafish Larva Model of Pentylenetetrazole-Induced Seizures. Zebrafish. 22:223229223-229.
Abstract
The use of zebrafish (Danio rerio) larva as an experimental model has gained a lot of interest in epilepsy research due to its multiple advantages over mammalian models. The present study investigated the time-dependent expression of c-fos, an immediate early gene, and a marker of neuronal activation, following pentylenetetrazole (PTZ)-induced seizures in zebrafish larvae at 7-day post-fertilization . The larvae were exposed to 8 mM PTZ for a 15-min period, transferred to fish system water, and processed for c-Fos expression analysis at 15, 30, 45, 60, and 90 min of the start of the experiment. c-fos mRNA and c-Fos protein levels were quantified, and Pearson correlation analysis was conducted to assess their relationship. PTZ exposure induced seizure-like behavior and resulted in a dynamic temporal expression of c-Fos, with both mRNA and protein achieving peak levels at 45 min and declining by 90 min. This approach applied a fixed exposure duration and defined post-exposure time points, which allowed a more accurate temporal profiling. The observed peak expression at 45 min suggested an optimal window for evaluating c-Fos expression in the PTZ-induced seizures model of zebrafish larva. These findings provided a valuable reference for selecting experimental endpoints in zebrafish larva seizure studies and enhanced the reliability of c-Fos as a marker of neuronal activation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping