PUBLICATION
S165F mutation-induced Junctophilin-2 autoinhibition impairs Ca2+ signaling in hypertrophic cardiomyopathy
- Authors
- Li, Z., Wang, J., Fang, X., Liu, H., Liu, W., Zhang, W., Li, J.
- ID
- ZDB-PUB-251126-13
- Date
- 2025
- Source
- Communications biology : (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- COS Cells
- Calcium Signaling*/genetics
- Cardiomyopathy, Hypertrophic*/genetics
- Cardiomyopathy, Hypertrophic*/metabolism
- Chlorocebus aethiops
- Humans
- Membrane Proteins*/chemistry
- Membrane Proteins*/genetics
- Membrane Proteins*/metabolism
- Muscle Proteins*/chemistry
- Muscle Proteins*/genetics
- Muscle Proteins*/metabolism
- Mutation*
- Rats
- Zebrafish
- PubMed
- 41291214 Full text @ Commun Biol
Citation
Li, Z., Wang, J., Fang, X., Liu, H., Liu, W., Zhang, W., Li, J. (2025) S165F mutation-induced Junctophilin-2 autoinhibition impairs Ca2+ signaling in hypertrophic cardiomyopathy. Communications biology. :.
Abstract
Hypertrophic cardiomyopathy (HCM) is a common inherited cardiac disorder often linked to mutations in various genes, including junctophilin-2 (JPH2), a key non-sarcomeric protein essential for forming junctional membrane complexes between the plasma membrane (PM) and endoplasmic/sarcoplasmic reticulum (ER/SR). Deciphering the molecular mechanisms behind these mutations is crucial for the development of effective therapies. In this study, we investigate the pathological effects of the HCM-associated S165F mutation in JPH2. Through integrated structural and biochemical analyses, we demonstrate that the S165F mutation induces an unintended intramolecular interaction in JPH2, disrupting its normal interaction with CaV1.2. This mutation compromises ER-PM junctions and disrupts Ca2+ signaling, leading to cellular hypertrophy in COS7 and H9c2 cell models. Furthermore, zebrafish overexpressing JPH2_S165F exhibit cardiac dysfunction, including pericardial edema and reduced heart rate. Notably, treatment with the ryanodine receptor agonist PCB-95 ameliorates these phenotypes, underscoring its potential clinical relevance. These findings offer new insights into the molecular mechanisms of JPH2-related HCM and provide a foundation for exploring novel therapeutic interventions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping