PUBLICATION
Mitochondrial Dysfunction Contributes to Decompensation in a Zebrafish Model of Isoproterenol-Induced Heart Failure
- Authors
- Vicente, M., García-Blázquez, A., Martínez-Sielva, A., Salgado-Almario, J., Jordán, J., Domingo, B., Llopis, J.
- ID
- ZDB-PUB-251101-1
- Date
- 2025
- Source
- Acta physiologica (Oxford, England) 241: e70128e70128 (Journal)
- Registered Authors
- Domingo Moreno, Beatriz, Llopis, Juan Francisco, Vicente Ruiz, Manuel
- Keywords
- ATP, calcium, heart failure, mitochondria, transcriptomics, zebrafish
- Datasets
- GEO:GSE278040
- MeSH Terms
-
- Adenosine Triphosphate/metabolism
- Animals
- Animals, Genetically Modified
- Calcium/metabolism
- Disease Models, Animal
- Heart Failure*/chemically induced
- Heart Failure*/metabolism
- Heart Failure*/physiopathology
- Isoproterenol*/toxicity
- Mitochondria, Heart*/metabolism
- Myocardial Contraction
- Zebrafish
- PubMed
- 41170837 Full text @ Acta Physiol. (Oxf).
Citation
Vicente, M., García-Blázquez, A., Martínez-Sielva, A., Salgado-Almario, J., Jordán, J., Domingo, B., Llopis, J. (2025) Mitochondrial Dysfunction Contributes to Decompensation in a Zebrafish Model of Isoproterenol-Induced Heart Failure. Acta physiologica (Oxford, England). 241:e70128e70128.
Abstract
Aim Heart failure is a clinical syndrome where the heart's structural or functional impairment leads to inadequate blood flow to meet the body's metabolic demands. Mitochondrial dysfunction is increasingly recognized as a central contributor underlying the contractile impairment observed in the failing heart. This study aimed to explore the interplay between calcium dynamics, cardiac mechanical performance, and mitochondrial ATP production during the progression of heart failure in zebrafish larvae exposed to chronic isoproterenol stimulation.
Methods Heart failure was induced by treating zebrafish larvae with 100 μM isoproterenol from 3 to 14 days postfertilization (dpf). Cardiac calcium transients, contractility, and mitochondrial ATP levels were assessed in vivo using transgenic lines expressing specific fluorescent biosensors. Additionally, transcriptomic analysis by RNA sequencing was performed on hearts collected at 14 dpf following prolonged isoproterenol exposure.
Results After 4 days of isoproterenol treatment (7 dpf), larvae exhibited ventricular dilation, reduced calcium levels, and diminished contractile force (p < 0.0001), although cardiac output remained intact. In contrast, extended treatment (11 days; 14 dpf) led to decompensated heart failure, characterized by a significant decline in cardiac output (p < 0.0001). Mitochondrial ATP levels were preserved at 7 dpf but dropped markedly at 14 dpf (p < 0.0001). Transcriptomic profiling at this later stage revealed downregulation of key functions (p < 0.05) involved in mitochondrial energy metabolism and energy transfer.
Conclusion In this model, heart dysfunction was initially evidenced by cardiac dilation. At 4 days of isoproterenol treatment, calcium levels and contractility decreased. Subsequently, decompensation coincided with a collapse in mitochondrial ATP production.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping