PUBLICATION
SIGIRR deficiency aggravates Mycobacterium marinum induced mortality and hepatic apoptosis in zebrafish
- Authors
- Yu, T., Chen, H., Yu, P., Hu, S., Luo, K., Gao, W., Yuan, H., Jingchen, Z., Xuefen, Y., Zhang, S., Xu, Q., Lian, Q.
- ID
- ZDB-PUB-251022-15
- Date
- 2025
- Source
- Developmental and comparative immunology : 105499105499 (Journal)
- Registered Authors
- Keywords
- M. marinum, SIGIRR, immune regulation, inflammatory response
- MeSH Terms
-
- Animals
- Apoptosis/genetics
- Liver*/immunology
- Liver*/pathology
- Mycobacterium Infections, Nontuberculous*/immunology
- Mycobacterium Infections, Nontuberculous*/mortality
- Mycobacterium marinum*/immunology
- Zebrafish*/immunology
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 41120022 Full text @ Dev. Comp. Immunol.
Citation
Yu, T., Chen, H., Yu, P., Hu, S., Luo, K., Gao, W., Yuan, H., Jingchen, Z., Xuefen, Y., Zhang, S., Xu, Q., Lian, Q. (2025) SIGIRR deficiency aggravates Mycobacterium marinum induced mortality and hepatic apoptosis in zebrafish. Developmental and comparative immunology. :105499105499.
Abstract
SIGIRR is a cell membrane protein in the TIR superfamily, widely expressed in tissues and organs. It has a unique structure and acts as a negative regulator of downstream inflammatory signaling pathways. Danio rerio (zebrafish) were experimentally infected with Mycobacterium marinum to investigate the role of SIGIRR in modulating host immune responses to bacterial infection. Following intraperitoneal injection of M. marinum, SIGIRR gene-deficient zebrafish exhibited an earlier onset of mortality compared to wild type, with the first death occurring sooner and all individuals dying by the fifth week. Wild-type zebrafish began dying in week two and all died by week seven, while SIGIRR-/- mutants died significantly faster. A zebrafish liver cell model was established, and apoptosis was measured by flow cytometry 24 hours post-infection. Apoptosis was 16% in wild-type cells and 25% in SIGIRR-/- mutant cells. The addition of SIGIRR polyclonal antibody to wild-type liver cells increased apoptosis to 18% after M. marinum challenge. Significant differences were observed among the three groups. These results show that SIGIRR critically suppresses the inflammatory response during bacterial infection.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping