PUBLICATION
Macrophage/microglia-dependent mechanisms drive retinal pigment epithelium regeneration in zebrafish
- Authors
- Leach, L.L., Gonzalez, R.G., Jayawardena, S.U., Gross, J.M.
- ID
- ZDB-PUB-250921-1
- Date
- 2025
- Source
- Cell Reports 44: 116325116325 (Journal)
- Registered Authors
- Keywords
- CP: Immunology, CSF1R, interleukin-34, macrophage, microglia, regeneration, retinal pigment epithelium, zebrafish
- MeSH Terms
-
- Animals
- Macrophages*/metabolism
- Microglia*/metabolism
- Phagocytosis
- Regeneration*
- Retinal Pigment Epithelium*/physiology
- Zebrafish*/physiology
- PubMed
- 40974576 Full text @ Cell Rep.
Citation
Leach, L.L., Gonzalez, R.G., Jayawardena, S.U., Gross, J.M. (2025) Macrophage/microglia-dependent mechanisms drive retinal pigment epithelium regeneration in zebrafish. Cell Reports. 44:116325116325.
Abstract
The retinal pigment epithelium (RPE) surrounds the posterior eye and maintains the health and function of the photoreceptors. Consequently, RPE dysfunction or damage has a devastating impact on vision. Due to complex etiologies, there are currently no cures for patients with RPE degenerative diseases, which remain some of the most prevalent causes of vision loss worldwide. Further, owing to a limited capacity for mammalian tissue repair, we know little about how the RPE regenerates. Here, we utilize zebrafish to uncover mechanisms driving intrinsic RPE regeneration. We show that microglia are indispensable for repair and present evidence that supports the role of interleukin-34 in recruiting macrophages/microglia to the RPE injury site. Further, we identify aberrant RPE injury site debris accumulation due to decreased macrophage/microglia localization, and we find that phagocytosis is an important mechanism driving debris clearance. Together, our results identify new regenerative functions of macrophages/microglia after RPE damage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping