PUBLICATION
egr1 and egr4 regulate zebrafish renal regeneration by promoting foxm1 expression
- Authors
- He, X., Sun, Y.
- ID
- ZDB-PUB-250816-5
- Date
- 2025
- Source
- Journal of molecular cell biology : (Journal)
- Registered Authors
- Sun, Yuhua
- Keywords
- foxm1, AKI, apoptosis, early growth response factors, proliferation, renal regeneration
- MeSH Terms
-
- Acute Kidney Injury*/chemically induced
- Acute Kidney Injury*/genetics
- Acute Kidney Injury*/metabolism
- Acute Kidney Injury*/pathology
- Animals
- Apoptosis/genetics
- Cell Proliferation/genetics
- Early Growth Response Protein 1*/genetics
- Early Growth Response Protein 1*/metabolism
- Forkhead Box Protein M1*/genetics
- Forkhead Box Protein M1*/metabolism
- Kidney*/metabolism
- Kidney*/physiology
- Promoter Regions, Genetic
- Regeneration*/genetics
- Zebrafish*/genetics
- Zebrafish*/physiology
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 40815265 Full text @ J. Mol. Cell Biol.
Citation
He, X., Sun, Y. (2025) egr1 and egr4 regulate zebrafish renal regeneration by promoting foxm1 expression. Journal of molecular cell biology. :.
Abstract
Early growth response (Egr) factors are involved in tissue development and repair. However, few studies have focused on the role of egr genes in renal regeneration after acute kidney injury (AKI) and the underlying mechanisms. In this study, we observed that egr1 and egr4 were sharply upregulated in wild type zebrafish at Day 1 post injury by gentamicin. Further experiments with egr1 and egr4 mutants showed that egr1 and egr4 were involved in zebrafish renal regeneration after AKI by regulating the proliferation and apoptosis of tubular cells. foxm1 is expressed in injured kidneys and involved in kidney repair. Here, loss of foxm1 inhibited zebrafish renal regeneration by decreasing proliferation and increasing apoptosis of tubular cells. Moreover, Egr1 and Egr4 promoted foxm1 expression by directly binding to the foxm1 promoter, thus regulating renal regeneration. Our results revealed that the rapid and transient induction of egr1 and egr4 after AKI exerts a reno-protective role through upregulating Foxm1 to facilitate kidney regeneration. Therefore, the egr1/egr4-foxm1 regulatory axis holds a therapeutic potential for the treatment of AKI.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping