PUBLICATION
In vivo antimicrobial activity of engineered mesoporous silica nanoparticles targeting intracellular mycobacteria
- Authors
- Aguilera-Correa, J.J., Tasrini, Y., Gisbert-Garzarán, M., Boulay, A., Carvalho, T., Blanchet, F.P., Vallet-Regí, M., Kremer, L.
- ID
- ZDB-PUB-250812-13
- Date
- 2025
- Source
- Nature communications 16: 7388 (Journal)
- Registered Authors
- Tasrini, Yara
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents*/administration & dosage
- Anti-Bacterial Agents*/chemistry
- Anti-Bacterial Agents*/pharmacology
- Biofilms/drug effects
- Humans
- Macrophages/drug effects
- Macrophages/microbiology
- Mycobacterium Infections, Nontuberculous*/drug therapy
- Mycobacterium Infections, Nontuberculous*/microbiology
- Mycobacterium marinum*/drug effects
- Nanoparticles*/chemistry
- Porosity
- Silicon Dioxide*/chemistry
- THP-1 Cells
- Zebrafish
- PubMed
- 40790109 Full text @ Nat. Commun.
Citation
Aguilera-Correa, J.J., Tasrini, Y., Gisbert-Garzarán, M., Boulay, A., Carvalho, T., Blanchet, F.P., Vallet-Regí, M., Kremer, L. (2025) In vivo antimicrobial activity of engineered mesoporous silica nanoparticles targeting intracellular mycobacteria. Nature communications. 16:7388.
Abstract
Treatments of Mycobacterium marinum, a common non-tuberculous mycobacterium associated with cutaneous infections are very challenging, emphasizing the development of new therapeutic approaches. Here we report the functionalization of mesoporous silica nanoparticles (MSN) with a series of triphenylphosphonium (TPP) substituents, which endowed them with affinity towards the surface of M. marinum in vitro, as well as within infected THP-1 cells. The presence of these nanoparticles at the bacterial surface prevents their uptake by human macrophages and dendritic cells. When loaded with doxycycline, the nanosystem exerts a potent anti-bacterial effect in planktonic cultures, biofilms, and in M. marinum-infected macrophages. Strikingly, in the M. marinum/zebrafish infection model, the doxycycline-loaded nanoparticles are associated with a pronounced decrease in the bacterial burden and a high embryo survival rate. These results disclose the proposed MSN nanosystems as a promising alternative for the treatment of M. marinum infection and, presumably, against a broader range of mycobacterial infections.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping