PUBLICATION
An effective fluorescent sensor for lipopolysaccharide-induced H2S detection and imaging in inflammatory cells, zebrafish, and mouse blood samples
- Authors
- Zou, X., Pang, M., He, J., Kang, Z., Wumaier, G., Xue, S., Yuan, L.
- ID
- ZDB-PUB-250724-10
- Date
- 2025
- Source
- RSC Advances 15: 258632587125863-25871 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 40697448 Full text @ RSC Adv.
Citation
Zou, X., Pang, M., He, J., Kang, Z., Wumaier, G., Xue, S., Yuan, L. (2025) An effective fluorescent sensor for lipopolysaccharide-induced H2S detection and imaging in inflammatory cells, zebrafish, and mouse blood samples. RSC Advances. 15:258632587125863-25871.
Abstract
In this work, a new fluorescent sensor (NAHCP) for the recognition of lipopolysaccharide-induced H2S detection and imaging in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples was constructed. Based on the o-aldehyde group (o-CHO) auxiliary 2,4-dinitrophenyl ether (DNP) group sulfide cleavage, this sensor shows high sensitivity and selectivity towards H2S, which can realize fluorescence "turn-on" detection. This sensor also features a very high response speed (∼2 min) and a large Stokes shift (113 nm) toward H2S. Additionally, the excellent detection limit (LOD) for H2S is as low as 25.6 nM. Impressively, this o-CHO auxiliary DNP group sulfide cleavage ensemble is further successfully applied for H2S detection in inflammatory Raw264.7 cells, zebrafish, and mouse blood samples.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping