PUBLICATION
A multi-tiered mechanical mechanism shapes the early neural plate
- Authors
- Inman, A., Spiritosanto, E., Evans, B.L., Lutton, J.E., Tada, M., Bretschneider, T., Haas, P.A., Smutny, M.
- ID
- ZDB-PUB-250705-15
- Date
- 2025
- Source
- Nature communications 16: 61876187 (Journal)
- Registered Authors
- Tada, Masazumi
- Keywords
- none
- MeSH Terms
-
- Mesoderm/embryology
- Gastrulation/physiology
- Gene Expression Regulation, Developmental
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*/embryology
- Embryo, Nonmammalian
- Neural Plate*/cytology
- Neural Plate*/embryology
- Neural Plate*/metabolism
- Biomechanical Phenomena
- Morphogenesis
- Cadherins/metabolism
- Animals
- Cell Movement
- PubMed
- 40615398 Full text @ Nat. Commun.
Citation
Inman, A., Spiritosanto, E., Evans, B.L., Lutton, J.E., Tada, M., Bretschneider, T., Haas, P.A., Smutny, M. (2025) A multi-tiered mechanical mechanism shapes the early neural plate. Nature communications. 16:61876187.
Abstract
The formation of complex tissues during embryonic development requires an intricate spatiotemporal coordination of local mechanical processes regulating global tissue morphogenesis. Here, we uncover a novel mechanism that mechanically regulates the shape of the anterior neural plate (ANP), a vital forebrain precursor, during zebrafish gastrulation. Combining in vivo and in silico approaches we reveal that the ANP is shaped by global tissue flows regulated by distinct force-generating processes. We show that mesendoderm migration and E-cadherin-dependent differential tissue interactions control distinct flow regimes in the neuroectoderm. Initial opposing flows lead to neuroectoderm cell internalisation and progressive multilayer tissue folding which in turn provide forces driving ANP tissue reshaping. We find that convergent extension is dispensable for internalisation but required for ANP tissue extension. Our results highlight how spatiotemporal regulation and coupling of different mechanical processes between tissues in the embryo control the first internalisation and folding events of the developing brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping