PUBLICATION
A large Stokes shift peptide-based fluorescent probe for Cd2+ and Hg2+ detection in aqueous solution and its applications in bioimaging, real sample, logic gate and smartphone device
- Authors
- Pu, C., Li, S., Cao, X., Chen, J., Wang, P., Wu, J.
- ID
- ZDB-PUB-250628-10
- Date
- 2025
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 343: 126599126599 (Journal)
- Registered Authors
- Keywords
- Cd(2+), Hg(2+), Large Stokes shift, Multifunctional application, Peptide-based probe
- MeSH Terms
-
- Animals
- Cadmium*/analysis
- Fluorescent Dyes*/chemistry
- Humans
- Limit of Detection
- Mercury*/analysis
- Optical Imaging/methods
- Oryza/chemistry
- Peptides*/chemistry
- Smartphone*
- Spectrometry, Fluorescence
- Water/chemistry
- Zebrafish
- PubMed
- 40577956 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Pu, C., Li, S., Cao, X., Chen, J., Wang, P., Wu, J. (2025) A large Stokes shift peptide-based fluorescent probe for Cd2+ and Hg2+ detection in aqueous solution and its applications in bioimaging, real sample, logic gate and smartphone device. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 343:126599126599.
Abstract
Realize the efficient and sensitive detection of cadmium ions (Cd2+) and mercury ions (Hg2+) based on different response modes is of great significance because their large emissions seriously harm the ecological environment and human health. Herein, a novel peptide-based fluorescent probe named DGGC was synthesized and characterized with satisfactory water solubility (100 % aqueous solution) and significantly large Stokes (230 nm). DGGC exhibited high selectivity and excellent sensitivity towards Cd2+ based on fluorescence enhancement response and Hg2+ based on fluorescence quenching response. The limit of detections (LODs) for Cd2+ and Hg2+ were as low as 8.6 nM and 35.1 nM, respectively. The results of fluorescence titration, Job's plot analysis and ESI-HRMS data revealed that DGGC combined with Cd2+ and Hg2+ in a 2:1 stoichiometric ratio. Additionally, the bioimaging results demonstrated that significant discrimination sensitivity of DGGC for imaging of Cd2+ and Hg2+ in living cells and zebrafish larvae. In addition, DGGC showed high precision and accuracy in the quantitative analysis of Cd2+ and Hg2+ in various real samples. Furthermore, DGGC monitored Cd2+ and Hg2+ contamination on the surface of rice, leaves, kiwifruit and pumpkin under 365 nm UV light using naked eyes. The fluorescence change of DGGC after the addition of Cd2+ and Hg2+ was used as an INHIBIT logic gate. More significantly, the smartphone colour recognition App was successfully applied to the semi-quantitative detection of Cd2+ and Hg2+ without the need for a large instrument.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping