PUBLICATION
Dynamic BMP signaling regulates sclerotome induction and lineage diversification in zebrafish
- Authors
- Xie, L., Ma, R.C., Kocha, K.M., Méndez-Olivos, E.E., Huang, P.
- ID
- ZDB-PUB-250605-14
- Date
- 2025
- Source
- Development (Cambridge, England) : (Journal)
- Registered Authors
- Huang, Peng, Kocha, Katrinka, Ma, Roger
- Keywords
- BMP signaling, Cell fate specification, Fibroblasts, Sclerotome, Somite, Zebrafish
- MeSH Terms
-
- Animals
- Bone Morphogenetic Protein 4*/genetics
- Bone Morphogenetic Protein 4*/metabolism
- Bone Morphogenetic Proteins*/genetics
- Bone Morphogenetic Proteins*/metabolism
- Cell Differentiation
- Cell Lineage*
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Mesoderm
- Notochord/cytology
- Notochord/embryology
- Notochord/metabolism
- Signal Transduction*/physiology
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- PubMed
- 40469047 Full text @ Development
Citation
Xie, L., Ma, R.C., Kocha, K.M., Méndez-Olivos, E.E., Huang, P. (2025) Dynamic BMP signaling regulates sclerotome induction and lineage diversification in zebrafish. Development (Cambridge, England). :.
Abstract
The sclerotome is an embryonic structure that gives rise to various supportive tissues, including the axial skeleton and connective tissues. Despite its significance, the mechanisms underlying sclerotome induction and diversification during embryonic development remain poorly understood. Sclerotome progenitors exhibit transient bmp4 expression and an active response to BMP signaling. Using BMP gain- and loss-of-function tools, we demonstrate that BMP signaling is both necessary and sufficient for sclerotome induction. Furthermore, through mosaic expression of a dominant-negative tool, we show that BMP signaling induces sclerotome fate in a cell-autonomous manner. Interestingly, different populations of sclerotome-derived cells have distinct BMP signaling requirements. Sclerotome-derived notochord-associated cells in the trunk lack BMP response, and sustained BMP signaling inhibits their differentiation into tenocytes. By contrast, sclerotome-derived fin mesenchymal cells in the fin fold require high levels of BMP signaling for proper morphogenesis. Our findings suggest that dynamic regulation of BMP signaling is crucial for the induction of the sclerotome and the subsequent diversification of sclerotome-derived lineages in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping