PUBLICATION
Zinc valproic acid complex promotes osteoblast differentiation and exhibits anti-osteoporotic potential
- Authors
- Wang, H., Xu, Y., Li, P., Wu, L.
- ID
- ZDB-PUB-250520-7
- Date
- 2025
- Source
- Open life sciences 20: 2025109020251090 (Journal)
- Registered Authors
- Keywords
- osteoporosis, rat osteoporosis model, valproic acid, zebrafish osteoporosis model, zinc
- MeSH Terms
- none
- PubMed
- 40391193 Full text @ Open Life Sci
Citation
Wang, H., Xu, Y., Li, P., Wu, L. (2025) Zinc valproic acid complex promotes osteoblast differentiation and exhibits anti-osteoporotic potential. Open life sciences. 20:2025109020251090.
Abstract
This study explores the potential of zinc valproic acid (Z-VA) complex as a promoter of osteoblast differentiation and a preventive agent against osteoporosis. The concentration of 25 µM Z-VA improved osteoblast differentiation by increasing the expression of Runx2 and type 1 collagen mRNA, alkaline phosphatase activity, and cellular calcium deposition. Dexamethasone-induced osteoporosis models were used in zebrafish and rats. In the zebrafish scale regeneration model, Z-VA decreased the hydroxyproline content and tartrate-resistant acid phosphatase activity while also upregulating the calcium to phosphorus molar ratio, Runx2a MASNA isoform, collagen2α, osteocalcin, and osteonectin. Additionally, Z-VA upregulated osteopontin and mitogen-activated protein kinase expression and downregulated matrix metalloproteinase 3 expression. Z-VA increased calcium deposition, callus formation, and bone growth in a zebrafish fracture model. In the rat model, Z-VA increased the bone transverse diameter, length, weight, mineral content, and mineral density, as well as serum Ca2+, inorganic phosphate, interleukin-6, tumor necrosis factor alpha, and alkaline phosphatase. Our results suggest that Z-VA may be an effective anti-osteoporotic agent that stimulates bone growth and prevents bone loss. However, further research is needed to elucidate its mechanisms and enhance its therapeutic application.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping