PUBLICATION
An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence
- Authors
- Fasano, G., Bonavolontà, V., Pedalino, C., Venditti, M., Paradisi, G., Petrini, S., Tartaglia, M., Lauri, A.
- ID
- ZDB-PUB-250520-3
- Date
- 2025
- Source
- Journal of visualized experiments : JoVE : (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- MAP Kinase Signaling System
- Animals
- Extracellular Signal-Regulated MAP Kinases*/antagonists & inhibitors
- Extracellular Signal-Regulated MAP Kinases*/metabolism
- Disease Models, Animal*
- Fluorescence Resonance Energy Transfer*/methods
- Noonan Syndrome*/enzymology
- Noonan Syndrome*/genetics
- Noonan Syndrome*/metabolism
- Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics
- Zebrafish/embryology
- Zebrafish/metabolism
- Microscopy, Fluorescence/methods
- PubMed
- 40388378 Full text @ J. Vis. Exp.
Citation
Fasano, G., Bonavolontà, V., Pedalino, C., Venditti, M., Paradisi, G., Petrini, S., Tartaglia, M., Lauri, A. (2025) An Efficient Protocol to Assess ERK Activity Modulation in Early Zebrafish Noonan Syndrome Models via Live FRET Microscopy and Immunofluorescence. Journal of visualized experiments : JoVE. :.
Abstract
RASopathies are genetic syndromes caused by ERK hyperactivation and resulting in multisystemic diseases that can also lead to cancer predisposition. Despite a broad genetic heterogeneity, germline gain-of-function mutations in key regulators of the RAS-MAPK pathway underlie the majority of the cases, and, thanks to advanced sequencing techniques, potentially pathogenic variants affecting the RAS-MAPK pathway continue to be identified. Functional validation of the pathogenicity of these variants, essential for accurate diagnosis, requires fast and reliable protocols, preferably in vivo. Given the scarcity of effective treatments in early childhood, such protocols, especially if scalable in cost-effective animal models, can be instrumental in offering a preclinical ground for drug repositioning/repurposing. Here we describe step-by-step the protocol for rapid generation of transient RASopathy models in zebrafish embryos and direct inspection of live disease-associated ERK activity changes occurring already during gastrulation through real-time multispectral Förster resonance energy transfer (FRET) imaging. The protocol uses a transgenic ERK reporter recently established and integrated with the hardware of commercial microscopes. We provide an example application for Noonan syndrome (NS) zebrafish models obtained by expression of the Shp2D61G. We describe a straightforward method that enables registration of ERK signal change in the NS fish model before and after pharmacological signal modulation by available low-dose MEK inhibitors. We detail how to generate, retrieve, and assess ratiometric FRET signals from multispectral acquisitions before and after treatment and how to cross-validate the results via classical immunofluorescence on whole embryos at early stages. We then describe how, via examining standard morphometric parameters, to query late changes in embryo shape, indicative of a resulting impairment of gastrulation, in the same embryos whose ERK activity is assessed by live FRET at 6 h post fertilization.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping