PUBLICATION
Colorimetric/fluorescent dual-channel recognition of L-tryptophan with rapid and visual sensing of HNQB-Cu(II) in zebrafish and nuts
- Authors
- Zhang, L., Yan, Y.B., Tong, L., Chen, R., Zhang, Y.
- ID
- ZDB-PUB-250519-1
- Date
- 2025
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 341: 126397126397 (Other)
- Registered Authors
- Keywords
- Cu(II) complex, Fluorescence sensor, L-tryptophan, Nut detection
- MeSH Terms
-
- Colorimetry*/methods
- Fluorescent Dyes*/chemistry
- Hydrazines*/chemistry
- Spectrometry, Fluorescence/methods
- Tryptophan*/analysis
- Copper*/chemistry
- Nuts*/chemistry
- Animals
- Zebrafish*/metabolism
- Limit of Detection
- PubMed
- 40381233 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Zhang, L., Yan, Y.B., Tong, L., Chen, R., Zhang, Y. (2025) Colorimetric/fluorescent dual-channel recognition of L-tryptophan with rapid and visual sensing of HNQB-Cu(II) in zebrafish and nuts. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 341:126397126397.
Abstract
In this paper, a fluorescent probe HNQB-Cu(II) was designed and synthesized for the rapid and intuitive detection of L-tryptophan. HNQB-Cu(II) was prepared from 2-hydroxy-N'-(quinolin-2-ylmethylene)benzohydrazide and copper(II) nitrate. The weak interaction forces of the HNQB-Cu(II) probe were studied by DFT calculation, electrostatic filament analysis, interaction region indicator (IRI) research and molecular freedom analysis. Then the fluorescence full scan and anti-interference experiments were carried out for 20 amino acids, and the test results manifested that the HNQB-Cu(II) probe has good selective identification performance and anti-interference ability for L-tryptophan. The sensor displayed good advantages in terms of recognition L-tryptophan with high specificity and selectivity, good time stability and low limit of detection (LOD = 3.9466 μM, R2 = 0.97802) in DMF. Combined HNQB-Cu(II) and paper a strip to detect L-tryptophan through a color change. Interestingly, HNQB-Cu(II) exhibits a superior fluorescence effect caudal fin of zebrafish a fluorescence microscope. In order to further research the application of check and measure L-tryptophan, the content of L-tryptophan in three kinds of nuts (pumpkin seeds, walnuts and peanuts) was quantitatively analyzed. The results show that HNQB-Cu(II) can reveal the L-tryptophan content more conveniently and quickly.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping