PUBLICATION
Prolonged 5-week and 12-week chronic stress differentially modulates CNS expression of pro- and anti-neuroinflammatory biomarkers, brain monoamines and affective behavior in adult zebrafish
- Authors
- Kotova, M.M., Amikishiev, S.V., Apukhtin, K.V., Galstyan, D.S., de Abreu, M.S., Stewart, A.M., Yang, L., Kalueff, A.V.
- ID
- ZDB-PUB-250413-3
- Date
- 2025
- Source
- Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology : (Journal)
- Registered Authors
- Keywords
- Anxiety, Chronic unpredictable stress, Depression, Neuroinflammation, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal
- Biogenic Monoamines*/metabolism
- Biomarkers/metabolism
- Brain*/metabolism
- Hydrocortisone/metabolism
- Male
- Microglia/metabolism
- Stress, Psychological*/metabolism
- Zebrafish*/metabolism
- Zebrafish*/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 40220038 Full text @ J. Comp. Physiol. B
Citation
Kotova, M.M., Amikishiev, S.V., Apukhtin, K.V., Galstyan, D.S., de Abreu, M.S., Stewart, A.M., Yang, L., Kalueff, A.V. (2025) Prolonged 5-week and 12-week chronic stress differentially modulates CNS expression of pro- and anti-neuroinflammatory biomarkers, brain monoamines and affective behavior in adult zebrafish. Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology. :.
Abstract
Chronic stress is a major cause of affective pathogenesis, such as anxiety and depression. Experimental animal models, including rodents and zebrafish, are a valuable tool for translational neuroscience research focusing on stress-related brain disorders. Here, we examined the effects of 5- and 12-week chronic unpredictable stress (CUS5 and CUS12) on zebrafish behavior, whole-body cortisol and neuroinflammation-related biomarker gene expression, including markers of pro-inflammatory microglia (NOS2a, COX2, P75NTR) and astroglia (C3, GBP), and markers of anti-inflammatory microglia (ARG-1, CD206) and astroglia (S100a10, PTX). We also assessed stress-induced changes in brain monoamine levels and brain-blood-barrier permeability. Overall, CUS5 induced anxiety-like behavior, accompanied by elevated CNS pro-inflammatory marker gene expression, cortisol signaling and norepinephrine levels. In contrast, CUS12 induced depression-like behavior, accompanied by lowered cortisol levels, impaired serotonin turnover and activated anti-inflammatory biomarker gene expression, as well as upregulated histone deacetylase 4 gene (suggesting the involvement of epigenetic regulation). Collectively, this confirms the importance of stress duration as a key factor in the development of stress-related disorders in zebrafish models, and further implicates pro- and inti-inflammatory neuroglia in affective pathogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping