PUBLICATION
tet2 and tet3 regulate cell fate specification and differentiation events during retinal development
- Authors
- Heilman, S.A., Schriever, H.C., Kostka, D., Koenig, K.M., Gross, J.M.
- ID
- ZDB-PUB-250327-19
- Date
- 2025
- Source
- Scientific Reports 15: 1040410404 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE283588
- MeSH Terms
-
- Cell Differentiation*/genetics
- Proto-Oncogene Proteins/genetics
- Proto-Oncogene Proteins/metabolism
- Cell Lineage/genetics
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Animals
- Zebrafish*/genetics
- Gene Expression Regulation, Developmental
- Dioxygenases*/genetics
- Dioxygenases*/metabolism
- DNA-Binding Proteins*/genetics
- DNA-Binding Proteins*/metabolism
- Retina*/metabolism
- PubMed
- 40140485 Full text @ Sci. Rep.
Citation
Heilman, S.A., Schriever, H.C., Kostka, D., Koenig, K.M., Gross, J.M. (2025) tet2 and tet3 regulate cell fate specification and differentiation events during retinal development. Scientific Reports. 15:1040410404.
Abstract
Tet family methylcytosine dioxygenases recognize and oxidize 5-methyl-cytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Previous work demonstrated the requirement for Tet and 5hmC during zebrafish retinogenesis. tet2-/-;tet3-/- mutants possessed defects in the formation of differentiated retinal neurons, but the mechanisms underlying these defects are unknown. Here, we leveraged scRNAseq technologies to better understand cell type-specific deficits and molecular signatures underlying the tet2-/-;tet3-/- retinal phenotype. Our results identified defects in tet2-/-;tet3-/- retinae that included delayed specification of several retinal cell types, reduced maturity across late-stage cones, expansions of immature subpopulations of horizontal and bipolar cells, and altered biases of bipolar cell subtype fates at late differentiation stages. Together, these data highlight the critical role that tet2 and tet3 play as regulators of cell fate specification and terminal differentiation events during retinal development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping