PUBLICATION
Fingerprint Analysis and Comparison of Activity Differences of Crude Venom from Five Species of Vermivorous Cone Snail in the South China Sea
- Authors
- Sun, S., Liao, Y., Fu, J., Liang, Y., Chen, Y., Mao, K., Gao, B.
- ID
- ZDB-PUB-250327-15
- Date
- 2025
- Source
- Marine drugs 23: (Journal)
- Registered Authors
- Keywords
- analgesic activities, anti-proliferative, cone snail, conotoxins, insecticidal activity
- MeSH Terms
-
- Animals
- Antineoplastic Agents/chemistry
- Antineoplastic Agents/pharmacology
- Conus Snail*
- Electron Transport Complex IV/genetics
- Humans
- Analgesics/chemistry
- Analgesics/pharmacology
- Conotoxins*/chemistry
- Conotoxins*/genetics
- Conotoxins*/pharmacology
- Zebrafish*
- Cell Line, Tumor
- Chromatography, High Pressure Liquid
- Lethal Dose 50
- Mollusk Venoms/chemistry
- China
- Insecticides*/chemistry
- Insecticides*/pharmacology
- PubMed
- 40137288 Full text @ Mar. Drugs
Citation
Sun, S., Liao, Y., Fu, J., Liang, Y., Chen, Y., Mao, K., Gao, B. (2025) Fingerprint Analysis and Comparison of Activity Differences of Crude Venom from Five Species of Vermivorous Cone Snail in the South China Sea. Marine drugs. 23:.
Abstract
The South China Sea is rich in cone snail resources, known for producing conotoxins with diverse biological activities such as analgesic, anticancer, and insecticidal effects. In this study, five vermivorous cone snail samples were collected from the South China Sea and their crude venom was extracted to investigate the variations in venom components and activities, aiming to identify highly active samples for further research. Cluster analysis using reverse-phase high-performance liquid chromatography (RP-HPLC) fingerprints and mitochondrial cytochrome c oxidase I (COI) gene sequences revealed that the diversity of venom components across different conotoxin species is genetically correlated. Activity assays demonstrated that all five cone snail venoms exhibited lethal effects on insects and zebrafish. Notably, the crude venom of Conus quercinus showed the highest insecticidal activity with an LD50 of 0.6 μg/mg, while C. tessellatus venom exhibited the most potent zebrafish lethality with an LD50 of 0.2 μg/mg. Furthermore, the crude venom from four cone snail species demonstrated toxicity against ovarian cancer cells, and only C. caracteristicu venom displayed significant analgesic activity. This study systematically identifies cone snail samples with promising insecticidal, anticancer, and analgesic properties, paving the way for the development and utilization of cone snail resources from the South China Sea and offering a novel approach for advancing marine peptide drug research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping