PUBLICATION
SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis
- Authors
- Bögershausen, N., Cavdarli, B., Nagai, T.H., Milev, M.P., Wolff, A., Mehranfar, M., Schmidt, J., Choudhary, D., Gutiérrez-Gutiérrez, Ó., Cyganek, L., Saint-Dic, D., Zibat, A., Köhrer, K., Wollenweber, T.E., Wieczorek, D., Altmüller, J., Borodina, T., Kaçar, D., Haliloğlu, G., Li, Y., Thiel, C., Sacher, M., Knapik, E.W., Yigit, G., Wollnik, B.
- ID
- ZDB-PUB-250326-5
- Date
- 2025
- Source
- JCI insight : (Journal)
- Registered Authors
- Choudhary, Dharmendra, Knapik, Ela W., Nagai, Taylor
- Keywords
- Epilepsy, Genetics, Glycobiology, Neuroscience, Protein traffic
- MeSH Terms
-
- Vesicular Transport Proteins*/deficiency
- Vesicular Transport Proteins*/genetics
- Vesicular Transport Proteins*/metabolism
- Female
- Epilepsy*/genetics
- Golgi Apparatus/metabolism
- Microcephaly/genetics
- Zebrafish
- Glycosylation
- Cataract*/genetics
- Cataract*/metabolism
- Animals
- Humans
- Male
- Pedigree
- Protein Transport
- PubMed
- 40131364 Full text @ JCI Insight
Citation
Bögershausen, N., Cavdarli, B., Nagai, T.H., Milev, M.P., Wolff, A., Mehranfar, M., Schmidt, J., Choudhary, D., Gutiérrez-Gutiérrez, Ó., Cyganek, L., Saint-Dic, D., Zibat, A., Köhrer, K., Wollenweber, T.E., Wieczorek, D., Altmüller, J., Borodina, T., Kaçar, D., Haliloğlu, G., Li, Y., Thiel, C., Sacher, M., Knapik, E.W., Yigit, G., Wollnik, B. (2025) SEC24C deficiency causes trafficking and glycosylation abnormalities in an epileptic encephalopathy with cataracts and dyserythropoeisis. JCI insight. :.
Abstract
As a major component of intracellular trafficking, the coat protein complex II (COPII) is indispensable for cellular function during embryonic development and throughout life. The four SEC24 proteins (A-D) are essential COPII components involved in cargo selection and packaging. A human disorder corresponding to alterations of SEC24 function is currently only known for SEC24D. Here, we report that biallelic loss of SEC24C leads to a syndrome characterized by primary microcephaly, brain anomalies, epilepsy, hearing loss, liver dysfunction, anemia, and cataracts in an extended consanguineous family with four affected individuals. We show that knockout of sec24C in zebrafish recapitulates important aspects of the human phenotype. SEC24C-deficient fibroblasts display alterations in the expression of several COPII components as well as impaired anterograde trafficking to the Golgi, indicating a severe impact on COPII function. Transcriptome analysis revealed that SEC24C deficiency also impacts the proteasome and autophagy pathways. Moreover, a shift in the N-glycosylation pattern and deregulation of the N-glycosylation pathway suggest a possible secondary alteration of protein glycosylation, linking the described disorder with the congenital disorders of glycosylation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping