PUBLICATION
Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration
- Authors
- Robertson, T.F., Schrope, J., Zwick, Z., Rindy, J., Horn, A., Hou, Y., Huttenlocher, A.
- ID
- ZDB-PUB-250322-6
- Date
- 2025
- Source
- Development (Cambridge, England) : (Journal)
- Registered Authors
- Huttenlocher, Anna
- Keywords
- Actin, Bleb, Cell migration, T cells, Tissues, Zebrafish
- MeSH Terms
-
- Actins/metabolism
- Animals
- Cell Movement*/physiology
- Epidermis/metabolism
- Keratinocytes/cytology
- Keratinocytes/metabolism
- Organ Specificity
- Pseudopodia/metabolism
- T-Lymphocytes*/cytology
- T-Lymphocytes*/physiology
- Zebrafish*/embryology
- PubMed
- 40114648 Full text @ Development
Citation
Robertson, T.F., Schrope, J., Zwick, Z., Rindy, J., Horn, A., Hou, Y., Huttenlocher, A. (2025) Live imaging in zebrafish reveals tissue-specific strategies for amoeboid migration. Development (Cambridge, England). :.
Abstract
Amoeboid cells like leukocytes can enter and migrate in diverse tissues, even though tissues vary widely in their chemical and mechanical composition. Here, we imaged motile T cells as they colonized peripheral tissues during zebrafish development to ask if cells tailor their migration strategy to their local tissue environment. We found that T cells in most sites migrated with F-actin-rich leading-edge pseudopods, matching how they migrate in vitro. T cells notably deviated from this strategy in the epidermis, where they instead migrated using a rearward concentration of F-actin and stable leading-edge blebs. This mode of migration occurs under planar confinement in vitro, and we found the stratified keratinocyte layers of the epidermis also impose planar-like confinement on leukocytes in vivo. Collectively, our data indicate that immune cells adapt their migration strategy to navigate different tissue geometries in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping