PUBLICATION
A novel easily available near-infrared fluorescent probe for rapid and specific detection of Au(Ⅲ) ion in environmental water samples and its biological imaging applications in living cells and zebrafish
- Authors
- Li, Z., Zhu, W., Tian, J., Liang, Y., Gong, S., Meng, Z., Xu, X., Wang, S., Wang, Z.
- ID
- ZDB-PUB-250310-16
- Date
- 2025
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 335: 125982125982 (Journal)
- Registered Authors
- Keywords
- Au(3+), Biological imaging, Fluorescent probe, Water samples
- MeSH Terms
-
- HeLa Cells
- Limit of Detection
- Optical Imaging/methods
- Animals
- Spectrometry, Fluorescence*/methods
- Water/chemistry
- Water Pollutants, Chemical*/analysis
- Fluorescent Dyes*/chemical synthesis
- Fluorescent Dyes*/chemistry
- Gold*/chemistry
- Humans
- Zebrafish*
- PubMed
- 40056879 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Li, Z., Zhu, W., Tian, J., Liang, Y., Gong, S., Meng, Z., Xu, X., Wang, S., Wang, Z. (2025) A novel easily available near-infrared fluorescent probe for rapid and specific detection of Au(Ⅲ) ion in environmental water samples and its biological imaging applications in living cells and zebrafish. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 335:125982125982.
Abstract
The in-situ tracking of Au3+ contaminant in the environment andorganisms has aroused considerable research interest. Herein, a simple colorimetric and near-infrared probe DPHB (2-3-(4-(dimethylamino)phenyl)allylidene)-6-hydroxybenzofuran-3(2H)-one) was strategically designed and readily synthesized for Au3+ monitoring. DPHB presented a conspicuous fluorescence quenching response toward Au3+ in nearly 100 % aqueous solution. DPHB was extremely sensitive and specific for Au3+ among different metal ions in the complex medium. This probe also possessed the the merits of large Stokes shift (180 nm), long emission wavelength (680 nm), low detection limit (85.23 nM), rapid response time (<4 s), favourable photostability, strong anti-interference ability, and good linear relationships of absorption intensity (R2 = 0.9902) and fluorescence intensity (R2 = 0.9952) versus Au3+ concentrations (0-5 μM). DPHB was successfully utilized for the accurate and precise determination of trace Au3+ within diverse environmental water samples. Furthermore, DPHB was is proved to be low-toxicity and could serve as an excellent fluorescence imaging marker for labeling the distribution of Au3+ levels in living HeLa cell and zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping