PUBLICATION
Toxicological Assessment of Ketamine in Juvenile Zebrafish (Danio rerio)
- Authors
- Tang, Y., Yang, K., Xu, J., Qiu, Y., Meng, L., Zhao, S.
- ID
- ZDB-PUB-250226-1
- Date
- 2025
- Source
- Toxics 13: (Journal)
- Registered Authors
- Keywords
- UPLC-LTQ/Orbitrap HRMS, behavior, ketamine, toxicity, zebrafish
- MeSH Terms
- none
- PubMed
- 39997899 Full text @ Toxics
Citation
Tang, Y., Yang, K., Xu, J., Qiu, Y., Meng, L., Zhao, S. (2025) Toxicological Assessment of Ketamine in Juvenile Zebrafish (Danio rerio). Toxics. 13:.
Abstract
This study investigates the toxic effects of ketamine on juvenile zebrafish, driven by increasing concerns over ketamine's prevalence and its potential neurotoxic effects that may disrupt behavior and metabolism. Employing a high-throughput behavior tracking system, the research analyzed the locomotor activity of 6-day post-fertilization (6 dpf) zebrafish exposed to various concentrations of ketamine. The integration of behavioral analysis with metabolic profiling was a notable innovation, as it establishes a comprehensive understanding of ketamine's effects on both acute behavioral inhibition and metabolic responses. The findings reveal that ketamine exposure significantly inhibits locomotor activity in juvenile zebrafish, with these effects becoming more pronounced at higher concentrations. Additionally, the detection of normethketamine, the primary metabolite of ketamine, using UPLC-LTQ/Orbitrap HRMS, confirms the zebrafish's ability to metabolize the drug. This underscores the utility of zebrafish as a model organism for studying the impact of ketamine on behavior and metabolism, providing valuable insights that may extend to other vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping