PUBLICATION
Identification of In Vivo and In Vitro Metabolites of Fentanyl Using UHPLC-Q-Orbitrap-HRMS
- Authors
- Liu, M., Zhao, S., Wang, B.J., Zhou, H., Liu, Y.
- ID
- ZDB-PUB-250216-7
- Date
- 2025
- Source
- Journal of analytical toxicology : (Journal)
- Registered Authors
- Keywords
- fentanyl, liver microsomes, metabolic pathways, metabolites, zebrafish
- MeSH Terms
-
- Analgesics, Opioid*/metabolism
- Animals
- Chromatography, High Pressure Liquid
- Fentanyl*/metabolism
- Mass Spectrometry
- Microsomes, Liver*/metabolism
- Zebrafish/metabolism
- PubMed
- 39953785 Full text @ J Anal Toxicol
Citation
Liu, M., Zhao, S., Wang, B.J., Zhou, H., Liu, Y. (2025) Identification of In Vivo and In Vitro Metabolites of Fentanyl Using UHPLC-Q-Orbitrap-HRMS. Journal of analytical toxicology. :.
Abstract
A comparative analysis of the metabolites and metabolic pathways of fentanyl was conducted in liver microsomes and zebrafish models utilizing ultra-high-performance liquid chromatography coupled with Q Exactive hybrid quadrupole-orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-HRMS). A total of 21 metabolites were identified across both liver microsomes and zebrafish models. These included 9 phase I metabolites, such as N-dealkylated, N-oxidated and hydroxylated products, and 12 phase II metabolites, including glucuronidated, methylated, and sulfated products, as well as a series of products derived from conjugation with glutathione. Notably, the products derived from conjugation with glutathione are reported here for the first time. This study provides a comprehensive and in-depth comparative analysis of fentanyl metabolism in liver microsomes and zebrafish, offering a foundation for analyzing and identifying biological samples in cases of fentanyl misuse and fatalities.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping