PUBLICATION
Ochratoxin A induces immunotoxicity by targeting Annexin A1 mediated neutrophil apoptosis in zebrafish
- Authors
- Zheng, Y., Liu, Y., Tian, J., Liu, S., Ma, G., Xie, Y., Zheng, C., Wu, Z.
- ID
- ZDB-PUB-250210-8
- Date
- 2025
- Source
- Frontiers in immunology 16: 15429641542964 (Journal)
- Registered Authors
- Keywords
- Annexin A1, aesculetin, apoptosis, immunotoxicity, ochratoxin A (OTA)
- MeSH Terms
-
- Animals
- Annexin A1*/metabolism
- Apoptosis*/drug effects
- Embryo, Nonmammalian/drug effects
- Immunity, Innate/drug effects
- Neutrophils*/drug effects
- Neutrophils*/immunology
- Neutrophils*/metabolism
- Ochratoxins*/toxicity
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 39925799 Full text @ Front Immunol
Citation
Zheng, Y., Liu, Y., Tian, J., Liu, S., Ma, G., Xie, Y., Zheng, C., Wu, Z. (2025) Ochratoxin A induces immunotoxicity by targeting Annexin A1 mediated neutrophil apoptosis in zebrafish. Frontiers in immunology. 16:15429641542964.
Abstract
Introduction Ochratoxin A (OTA) is a toxic secondary metabolite produced by Aspergillus and Penicillium species, posing a significant threat to global food safety. Previous studies have demonstrated the diverse toxic effects of OTA, including hepatotoxicity, nephrotoxicity, and carcinogenicity. However, limited understanding exists regarding its immunotoxicity and the underlying mechanisms, particularly in relation to innate immunity.
Methods Zebrafish embryos were exposed to varying concentrations of OTA to assess its impact on embryonic development, innate immune cell formation, and immune response. Transcriptome sequencing analysis was performed to identify changes in gene expression. Additionally, the potential therapeutic effect of aesculetin was evaluated.
Results Our results demonstrated that exposure to OTA inhibited embryonic development and induced malformations in a concentration-dependent manner. Additionally, OTA exposure led to a significant reduction in the number of neutrophils and macrophages, indicating compromised formation of innate immune cells. Furthermore, OTA exposure hampered the immune response during zebrafish fin regeneration, as evidenced by the diminished migration of neutrophils and macrophages to the wound area. Transcriptome sequencing analysis identified significant up-regulation of the anxa1a and anxa1d-mediated apoptosis signaling pathway in neutrophils following OTA treatment. Notably, administration of aesculetin, known for its anti-apoptosis activity, effectively attenuated the immunotoxic effects induced by OTA.
Discussion These findings provide valuable insights into the immunotoxicity of OTA while highlight the potential therapeutic strategy using aesculetin for mitigating immune dysfunction caused by OTA.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping