PUBLICATION
Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes
- Authors
- Arnold, H., Panara, V., Allalou, A., Koltowska, K.
- ID
- ZDB-PUB-250210-3
- Date
- 2025
- Source
- STAR protocols 6: 103632103632 (Journal)
- Registered Authors
- Panara, Virginia
- Keywords
- bioinformatics, developmental biology, model organisms
- MeSH Terms
-
- Embryo, Nonmammalian
- Microscopy, Confocal*/methods
- Phenotype*
- Zebrafish*/embryology
- Animals
- Image Processing, Computer-Assisted*/methods
- Lymphatic Vessels*/diagnostic imaging
- PubMed
- 39923241 Full text @ STAR Protoc
Citation
Arnold, H., Panara, V., Allalou, A., Koltowska, K. (2025) Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes. STAR protocols. 6:103632103632.
Abstract
Characterization of zebrafish mutants is routinely time consuming as morphological phenotypes can be subtle or variable, thus hampering robust quantification. Here, we present a protocol to identify the overall phenotype by overlaying images of lymphatic vessels acquired from multiple zebrafish embryos of a genetic background. We describe steps for preparing samples, obtaining confocal images, and their computational alignment. In addition, we supply sample images for testing. For complete details on the use and execution of this protocol, please refer to Arnold et al.1.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping