PUBLICATION
Discovery of specific activity of 2-hydroxypentanoic acid acting on the mPR alpha (Paqr7) from the marine algae Padina
- Authors
- Amin, M.T., Acharjee, M., Sarwar Jyoti, M.M., Rezanujjaman, M., Hassan, M.M., Hossain, M.F., Ahamed, S., Kodani, S., Tokumoto, T.
- ID
- ZDB-PUB-250209-9
- Date
- 2025
- Source
- Biochemical and Biophysical Research Communications 751: 151433151433 (Journal)
- Registered Authors
- Tokumoto, Toshinobu
- Keywords
- 2-Hydroxypentanoic acid, Antagonist, Marine algae, Membrane progesterone receptor, Oocyte maturation, Padina
- MeSH Terms
-
- Zebrafish
- Humans
- Oocytes/drug effects
- Oocytes/metabolism
- Receptors, Progesterone*/antagonists & inhibitors
- Receptors, Progesterone*/metabolism
- Animals
- Female
- PubMed
- 39922053 Full text @ Biochem. Biophys. Res. Commun.
Citation
Amin, M.T., Acharjee, M., Sarwar Jyoti, M.M., Rezanujjaman, M., Hassan, M.M., Hossain, M.F., Ahamed, S., Kodani, S., Tokumoto, T. (2025) Discovery of specific activity of 2-hydroxypentanoic acid acting on the mPR alpha (Paqr7) from the marine algae Padina. Biochemical and Biophysical Research Communications. 751:151433151433.
Abstract
Membrane progesterone receptors (mPRs) are members of the progestin and adipoQ (PAQR) receptor family that are stimulated by endogenous steroids to initiate rapid intracellular signaling through a nongenomic pathway. Previously, water-soluble compounds with mPRα binding activity from the marine algae Padina arborescens were fractionated by HPLC. Nuclear magnetic resonance spectroscopy revealed that the major component of the HPLC fraction was 2-hydroxypentanoic acid (2-HPA). In this study, the physiological activity of 2-HPA and its analogues was investigated using in vitro goldfish and in vivo zebrafish oocyte maturation and ovulation assays. Only 2-HPA showed inhibitory activity on oocyte maturation and ovulation of fish oocytes. The inhibitory activity of 2-HPA was compared between S- and R-type 2-HPA. The results showed that both types had the same level of activity. Furthermore, the interaction of 2-HPA with mPRα was analyzed by binding assay. 2-HPAs showed a substantial competitive binding affinity for the human membrane progesterone receptor α (hmPRα) in the graphene quantum dots (GQDs)-hmPRα binding assay. In contrast, synthetic structural analogues of 2-HPA showed no competitive binding activity. These results indicate that 2-HPA is a novel mPRα antagonist, and its chemical structure is highly restricted to show its activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping