PUBLICATION
Exploring the Anti-Adherence Potential of Skt35 to Combat Catheter-Associated Staphylococcus aureus Infections: Efficacy, Toxicity and Mechanism of Action
- Authors
- Meenatchi, R., Priya, S., Shreya, C., Gopi, S., Rajagopal, R., Kaliraj, S., Kumaradoss, K.M., Arockiaraj, J.
- ID
- ZDB-PUB-250121-22
- Date
- 2025
- Source
- Chemistry & biodiversity : e202402087e202402087 (Journal)
- Registered Authors
- Keywords
- Staphylococcus aureus, antibacterial, antibiofilm, catheter‐associated urinary tract infections (CAUTI), dioxopiperidinamide
- MeSH Terms
-
- Animals
- Anti-Bacterial Agents*/chemistry
- Anti-Bacterial Agents*/pharmacology
- Bacterial Adhesion*/drug effects
- Biofilms/drug effects
- Catheter-Related Infections*/drug therapy
- Catheter-Related Infections*/microbiology
- Cinnamates*/chemistry
- Cinnamates*/pharmacology
- Dose-Response Relationship, Drug
- Humans
- Microbial Sensitivity Tests
- Molecular Docking Simulation
- Molecular Structure
- Quorum Sensing/drug effects
- Staphylococcal Infections*/drug therapy
- Staphylococcal Infections*/microbiology
- Staphylococcus aureus*/drug effects
- Structure-Activity Relationship
- Zebrafish
- PubMed
- 39832262 Full text @ Chem. Biodivers.
Citation
Meenatchi, R., Priya, S., Shreya, C., Gopi, S., Rajagopal, R., Kaliraj, S., Kumaradoss, K.M., Arockiaraj, J. (2025) Exploring the Anti-Adherence Potential of Skt35 to Combat Catheter-Associated Staphylococcus aureus Infections: Efficacy, Toxicity and Mechanism of Action. Chemistry & biodiversity. :e202402087e202402087.
Abstract
Catheter-associated urinary tract infections (CAUTIs), often caused by biofilm-forming Staphylococcus aureus, present significant clinical challenges. Skt35, a dioxopiperidinamide derivative of cinnamic acid, was investigated for its potential antibacterial and antibiofilm activities against S. aureus biofilms. The antibacterial effect of Skt35 was assessed using the zone of inhibition and microdilution methods, revealing a minimum inhibitory concentration (MIC) of 250 µM. Antibiofilm properties were confirmed through crystal violet assays, scanning electron microscopy and confocal laser scanning microscopy, showing significant biofilm inhibition at the Sub-MIC. In an in vitro bladder model, Skt35-coated silicone catheter tubes exhibited significant antiadhesive effects. Zebrafish embryo tests indicated no toxicity at concentrations up to 125 µM. Molecular docking and simulation analysis revealed strong binding affinities of Skt35 to Accessory Gene Regulator A (-7.9 kcal/mol) and Lux Small protein (-4.96 kcal/mol), suggesting potential disruption of quorum sensing and gene expression in S. aureus, making it a promising candidate for catheter coatings to prevent CAUTIs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping