PUBLICATION
Zebrafish glial-vascular interactions progressively expand over the course of brain development
- Authors
- Gall, L.G., Stains, C.M., Freitas-Andrade, M., Jia, B.Z., Patel, N., Megason, S.G., Lacoste, B., O'Brown, N.M.
- ID
- ZDB-PUB-250115-8
- Date
- 2024
- Source
- iScience 28: 111549 (Journal)
- Registered Authors
- Megason, Sean, O'Brown, Natasha
- Keywords
- Biological sciences, Developmental biology, Neuroscience
- MeSH Terms
- none
- PubMed
- 39811646 Full text @ iScience
Citation
Gall, L.G., Stains, C.M., Freitas-Andrade, M., Jia, B.Z., Patel, N., Megason, S.G., Lacoste, B., O'Brown, N.M. (2024) Zebrafish glial-vascular interactions progressively expand over the course of brain development. iScience. 28:111549.
Abstract
Glial-vascular interactions are critical for the formation and maintenance of brain blood vessels and the blood-brain barrier (BBB) in mammals, but their role in the zebrafish BBB remains unclear. Using three glial gene promoters-gfap, glast, and glastini (a truncated glast)-we explored glial-vascular development in zebrafish. Sparse labeling showed fewer glial-vascular interactions at early stages, with glial coverage and contact area increasing with age. Stable transgenic lines for glast and glastini revealed similar developmental increases, starting at ∼30% coverage at 3 days post-fertilization (dpf) and peaking at ∼60% by 10 dpf, and consistently higher glial coverage in the forebrain and midbrain than in the hindbrain. Electron microscopy analyses showed similar progressive increases in glial-vascular interactions, with maximal coverage of ∼70% in adults-significantly lower than the ∼100% seen in mammals. These findings define the temporal and regional maturation of glial-vascular interactions in zebrafish and highlight differences from mammalian systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping