PUBLICATION
A Novel Organophosphate Ester, Tris(2,4-ditert-butylphenyl) Phosphate, Induced Reproductive Toxicity in Male Zebrafish at Environmentally Relevant Concentrations
- Authors
- Zhang, Y., Qin, H., Li, B., Yu, Z., Zu, B., Kong, R., Letcher, R.J., Liu, C., Zhou, B.
- ID
- ZDB-PUB-250109-44
- Date
- 2024
- Source
- Environmental science & technology 59(1): 279-290 (Journal)
- Registered Authors
- Zhou, BingSheng
- Keywords
- nuclear receptor, organophosphate ester, reproductive toxicity, tris(2,4-ditert-butylphenyl) phosphate, vitamin D, zebrafish
- MeSH Terms
-
- Reproduction/drug effects
- Zebrafish*
- Animals
- Male
- Testis/drug effects
- Testis/metabolism
- Water Pollutants, Chemical/toxicity
- Organophosphates*/toxicity
- Spermatozoa/drug effects
- PubMed
- 39718999 Full text @ Env. Sci. Tech.
Citation
Zhang, Y., Qin, H., Li, B., Yu, Z., Zu, B., Kong, R., Letcher, R.J., Liu, C., Zhou, B. (2024) A Novel Organophosphate Ester, Tris(2,4-ditert-butylphenyl) Phosphate, Induced Reproductive Toxicity in Male Zebrafish at Environmentally Relevant Concentrations. Environmental science & technology. 59(1):279-290.
Abstract
As a novel organophosphate ester (NOPE), tris(2,4-ditert-butylphenyl) phosphate (TDtBPP) has attracted significant attention due to its unexpectedly high detection in natural environments. However, the ecological toxic effects of environmentally relevant concentrations of TDtBPP in organisms remain entirely unknown. In this study, 1 month old zebrafish were exposed to 0, 50, 500, or 5000 ng/L TDtBPP for 150 days, and the reproductive toxicity in male fish was evaluated. Results demonstrated that TDtBPP exposure significantly inhibited the maturation of spermatozoa and thus decreased spermatogenesis. Furthermore, abnormal sperm morphology and decreased sperm motility were also observed. The decrease in sperm quantity and quality eventually resulted in the declining fecundity. Moreover, TDtBPP exposure downregulated the expression of hsd3b1 in vivo and in vitro and subsequently inhibited the synthesis of androgens in zebrafish testes and Leydig cells. This inhibition of androgen synthesis appeared to be responsible for the observed reproductive toxicity in male fish. Molecular docking and dual-luciferase reporter gene experiments elucidated that TDtBPP inhibited the promotion of vitamin D on hsd3b1 transcription by the vitamin D receptor and thus downregulated the expression of hsd3b1. Our findings provide first time evidence that TDtBPP poses a risk to male fish reproduction at environmentally relevant levels.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping