PUBLICATION
In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy
- Authors
- Li, Y., Xu, X., Zhang, C., Sun, X., Zhou, S., Li, X., Guo, J., Hu, R., Qu, J., Liu, L.
- ID
- ZDB-PUB-250109-37
- Date
- 2024
- Source
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) : e2410605e2410605 (Journal)
- Registered Authors
- Keywords
- frequency‐domain fluorescence lifetime microscopy, high‐speed volumetric projection imaging, in vivo neurodynamics
- MeSH Terms
-
- Animals
- Brain*/diagnostic imaging
- Calcium/metabolism
- Imaging, Three-Dimensional/methods
- Mice
- Microglia/metabolism
- Microglia/physiology
- Microscopy, Fluorescence, Multiphoton/methods
- Neurons/metabolism
- Neurons/physiology
- Zebrafish*
- PubMed
- 39716869 Full text @ Adv Sci (Weinh)
Citation
Li, Y., Xu, X., Zhang, C., Sun, X., Zhou, S., Li, X., Guo, J., Hu, R., Qu, J., Liu, L. (2024) In Vivo Neurodynamics Mapping via High-Speed Two-Photon Fluorescence Lifetime Volumetric Projection Microscopy. Advanced science (Weinheim, Baden-Wurttemberg, Germany). :e2410605e2410605.
Abstract
Monitoring the morphological and biochemical information of neurons and glial cells at high temporal resolution in three-dimensional (3D) volumes of in vivo is pivotal for understanding their structure and function, and quantifying the brain microenvironment. Conventional two-photon fluorescence lifetime volumetric imaging speed faces the acquisition speed challenges of slow serial focal tomographic scanning, complex post-processing procedures for lifetime images, and inherent trade-offs among contrast, signal-to-noise ratio, and speed. This study presents a two-photon fluorescence lifetime volumetric projection microscopy using an axially elongated Bessel focus and instant frequency-domain fluorescence lifetime technique, and integrating with a convolutional network to enhance the imaging speed for in vivo neurodynamics mapping. The proposed method is validated by monitoring intracellular Ca2+ concentration throughout whole volume, tracking microglia movement and microenvironmental changes following thermal injury in the zebrafish brain, analyzing structural and functional variations of gap junctions in astrocyte networks, and measuring the Ca2+ concentration in neurons in mouse brains. This innovative methodology enables quantitative in vivo visualization of neurodynamics and the cellular processes and interactions in the brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping