PUBLICATION
Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development
- Authors
- Tignard, P., Pottin, K., Geeverding, A., Doulazmi, M., Cabrera, M., Fouquet, C., Liffran, M., Fouchard, J., Rosello, M., Albadri, S., Del Bene, F., Trembleau, A., Breau, M.A.
- ID
- ZDB-PUB-250109-30
- Date
- 2024
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Albadri, Shahad, Breau, Marie, Del Bene, Filippo, Rosello, Marion
- Keywords
- axon, basement membrane, developmental biology, extracellular matrix, morphogenesis, olfactory, placode, zebrafish
- MeSH Terms
-
- Basement Membrane*/metabolism
- Zebrafish*/embryology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Olfactory Bulb/embryology
- Olfactory Bulb/metabolism
- Axons*/metabolism
- Axons*/physiology
- Olfactory Mucosa/embryology
- Olfactory Mucosa/metabolism
- Animals
- Laminin/metabolism
- Ectodermal Placodes
- Mutation
- Brain/embryology
- Brain/metabolism
- Morphogenesis
- PubMed
- 39713923 Full text @ Elife
Citation
Tignard, P., Pottin, K., Geeverding, A., Doulazmi, M., Cabrera, M., Fouquet, C., Liffran, M., Fouchard, J., Rosello, M., Albadri, S., Del Bene, F., Trembleau, A., Breau, M.A. (2024) Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development. eLIFE. 12:.
Abstract
Despite recent progress, the complex roles played by the extracellular matrix in development and disease are still far from being fully understood. Here, we took advantage of the zebrafish sly mutation which affects Laminin γ1, a major component of basement membranes, to explore its role in the development of the olfactory system. Following a detailed characterisation of Laminin distribution in the developing olfactory circuit, we analysed basement membrane integrity, olfactory placode and brain morphogenesis, and olfactory axon development in sly mutants, using a combination of immunochemistry, electron microscopy and quantitative live imaging of cell movements and axon behaviours. Our results point to an original and dual contribution of Laminin γ1-dependent basement membranes in organising the border between the olfactory placode and the adjacent brain: they maintain placode shape and position in the face of major brain morphogenetic movements, they establish a robust physical barrier between the two tissues while at the same time allowing the local entry of the sensory axons into the brain and their navigation towards the olfactory bulb. This work thus identifies key roles of Laminin γ1-dependent basement membranes in neuronal tissue morphogenesis and axon development in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping