PUBLICATION
Expansion microscopy reveals neural circuit organization in genetic animal models
- Authors
- Behzadi, S., Ho, J., Tanvir, Z., Haspel, G., Freifeld, L., Severi, K.E.
- ID
- ZDB-PUB-250109-27
- Date
- 2025
- Source
- Neurophotonics 12: 010601010601 (Review)
- Registered Authors
- Severi, Kristen, Tanvir, Zainab
- Keywords
- connectomics, expansion microscopy, genetic models, neural circuits, resolution
- MeSH Terms
- none
- PubMed
- 39712646 Full text @ Neurophotonics
Citation
Behzadi, S., Ho, J., Tanvir, Z., Haspel, G., Freifeld, L., Severi, K.E. (2025) Expansion microscopy reveals neural circuit organization in genetic animal models. Neurophotonics. 12:010601010601.
Abstract
Expansion microscopy is a super-resolution technique in which physically enlarging the samples in an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolved using light microscopy. This is particularly useful in neuroscience as many important structures are smaller than the diffraction limit. Since its invention in 2015, a variety of expansion microscopy protocols have been generated and applied to advance knowledge in many prominent organisms in neuroscience, including zebrafish, mice, Drosophila, and Caenorhabditis elegans. We review the last decade of expansion microscopy-enabled advances with a focus on neuroscience.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping