PUBLICATION
Selenocysteine-Activatable Near-Infrared Fluorescent Probe for Screening of Anti-inflammatory Components in Herbs
- Authors
- Liu, H., Zhang, W., Wang, S., Zhou, Q., Xu, N., Zhang, W., Ren, H., Yang, M., Lu, H., Zheng, X., Tian, J.
- ID
- ZDB-PUB-250109-150
- Date
- 2025
- Source
- Analytical chemistry 97(1): 75-85 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents*/chemical synthesis
- Anti-Inflammatory Agents*/chemistry
- Anti-Inflammatory Agents*/pharmacology
- Anti-Inflammatory Agents*/therapeutic use
- Drug Evaluation, Preclinical
- Fluorescent Dyes*/chemical synthesis
- Fluorescent Dyes*/chemistry
- Humans
- Inflammation/diagnostic imaging
- Inflammation/drug therapy
- Infrared Rays
- Mice
- Optical Imaging
- RAW 264.7 Cells
- Selenocysteine*/analysis
- Selenocysteine*/chemistry
- Zebrafish*
- PubMed
- 39754546 Full text @ Anal. Chem.
Citation
Liu, H., Zhang, W., Wang, S., Zhou, Q., Xu, N., Zhang, W., Ren, H., Yang, M., Lu, H., Zheng, X., Tian, J. (2025) Selenocysteine-Activatable Near-Infrared Fluorescent Probe for Screening of Anti-inflammatory Components in Herbs. Analytical chemistry. 97(1):75-85.
Abstract
Inflammation, a central process in numerous diseases, plays a crucial role in hepatic disorders, arthritis, cardiac conditions, and neurodegenerative ailments. Given the lack of effective anti-inflammatory drugs, it is imperative to assess inflammation severity and explore novel therapeutics. Selenocysteine (Sec), a key mediator of selenium's biological function, is closely involved in anti-inflammatory responses. We have synthesized a novel near-infrared fluorescent probe, Sec-BDP, which can image Sec dynamics in vivo with high selectivity and sensitivity. Sec-BDP detects Sec at concentrations as low as 0.085 μM. Utilizing this probe, we visualized Sec levels in cell, zebrafish, and mouse inflammation models, enabling a clear assessment of inflammation severity. To screen for drug candidates, Sec-BDP was integrated with ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry to identify potent anti-inflammatory compounds in Astragalus membranaceus, such as 5-O-methylvisammioside. Imaging of Sec with Sec-BDP provides insights into Sec-related diseases and aids in discovering new treatments. This probe advances selenium biology and promises more targeted therapeutic strategies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping