PUBLICATION
A turn-on mitochondria-targeted iridium (Ⅲ) Complex-Based probe for glutathione detection and photodynamic therapy of cancer cells
- Authors
- Mao, X., Fei, X., Cai, T., Xu, S., Zhang, D., Pu, S., Li, Z.
- ID
- ZDB-PUB-241218-18
- Date
- 2024
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 329: 125579 (Journal)
- Registered Authors
- Keywords
- Bioimaging, Fluorescent probe, Glutathione, Iridium complex, Single-linear oxygen
- MeSH Terms
-
- Animals
- Cell Line, Tumor
- Coordination Complexes/chemical synthesis
- Coordination Complexes/chemistry
- Coordination Complexes/pharmacology
- Fluorescent Dyes*/chemistry
- Fluorescent Dyes*/pharmacology
- Glutathione*/metabolism
- HeLa Cells
- Humans
- Iridium*/chemistry
- Iridium*/pharmacology
- Mitochondria*/drug effects
- Mitochondria*/metabolism
- Neoplasms/drug therapy
- Neoplasms/pathology
- Photochemotherapy*/methods
- Photosensitizing Agents/chemistry
- Photosensitizing Agents/pharmacology
- Spectrometry, Fluorescence
- Zebrafish*
- PubMed
- 39689545 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Mao, X., Fei, X., Cai, T., Xu, S., Zhang, D., Pu, S., Li, Z. (2024) A turn-on mitochondria-targeted iridium (Ⅲ) Complex-Based probe for glutathione detection and photodynamic therapy of cancer cells. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 329:125579.
Abstract
As one of the most abundant biothiols in cells, glutathione (GSH) usually exists in a dynamic equilibrium of oxidized glutathione (GSSG) and reduces glutathione redox, and plays an essential reducing substance to maintain the REDOX balance of the microenvironment. So, the development of a reliable GSH sensor will be important for living cells and organisms. We fabricated a mitochondria targeted "turn-on" fluorescent sensor based on Ir (III) complex and successfully detected endogenous and exogenous GSH in living cells and zebrafish. For the probe Ir-DINI, a robust electron-withdrawing group 2,4-dinitrobenzoyl was introduced to quench the fluorescence, which could be broken through electrostatic interaction with GSH, following exposing a strong fluorescent Ir (Ⅲ) complex Ir-OH. On the other hand, photodynamic therapy (PDT) has attracted much attention in recent years due to its minimally invasive treatment. We found that singlet oxygen yields of probe Ir-DINI displayed an enhancement before and after the detection of GSH. Additionally, photodynamic studies in living cells illustrated that after reacting with GSH, probe Ir-DINI exhibited more obvious phototoxicity than before the detection of GSH. So the probe Ir-DINI could be served as a GSH sensor and potential GSH-activated photosensitizer for photodynamic therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping