PUBLICATION
520 nm and 660 nm light-emitting diodes modulates pancreatic development and beta cell functions in zebrafish embryos
- Authors
- Üstündağ, Ü.V., Ünal, İ., Cansız, D., Beler, M., Kanagaraj, N.K., Kumar, A.R., Peravali, R., Emekli-Alturfan, E.
- ID
- ZDB-PUB-241210-12
- Date
- 2024
- Source
- Photochemistry and photobiology : (Journal)
- Registered Authors
- Beler, Merih, Emekli-Alturfan, Ebru, Peravali, Ravindra, Unal, Ismail, Üstündağ, Ünsal Veli
- Keywords
- beta cell, insulin, light emitting diode, pancreas, zebrafish
- MeSH Terms
-
- Insulin/metabolism
- Insulin-Secreting Cells*/cytology
- Insulin-Secreting Cells*/metabolism
- Insulin-Secreting Cells*/radiation effects
- Embryo, Nonmammalian/radiation effects
- Pancreas*/embryology
- Pancreas*/metabolism
- Pancreas*/radiation effects
- Circadian Rhythm/radiation effects
- Glutathione/metabolism
- Oxidative Stress/radiation effects
- Animals
- Zebrafish*/embryology
- Malondialdehyde/metabolism
- Light*
- PubMed
- 39655413 Full text @ Photochem. Photobiol.
Citation
Üstündağ, Ü.V., Ünal, İ., Cansız, D., Beler, M., Kanagaraj, N.K., Kumar, A.R., Peravali, R., Emekli-Alturfan, E. (2024) 520 nm and 660 nm light-emitting diodes modulates pancreatic development and beta cell functions in zebrafish embryos. Photochemistry and photobiology. :.
Abstract
Green and Red LEDs increase insulin production, but their comparative effects on pancreatic and beta cell development are unclear. Zebrafish embryos were divided into three groups: Control (n = 60), Green (G) (n = 60), and Red (R) (n = 60), then irradiated for three days (14 hours/day) with 0.5 W/cm2 G (λpeak = 520 nm, 180 mA) and R (λpeak = 660 nm, 210 mA). At the end of 72 h, pancreatic and beta cells, circadian rhythm, and oxidative stress gene were analyzed using RT-PCR. Malondialdehyde, nitric oxide, superoxide dismutase, and glutathione levels were also evaluated. In the Red group, pancreatic area increased by ~97.13% compared to the Control group and by approximately ~62.16% compared to the G group (both p < 0.0001), and no significant difference in beta cell area (p = 0.964). G group insulin expression increased 2.31-fold compared to R group (p < 0.0001). Red LED treatment increased MDA levels (p < 0.001), oxidative stress (fth1b, nqo1) (p < 0.0001), and per1b during the photophase (p < 0.0001) compared to G group. R LED treatment increases oxidative stress and disrupts circadian rhythm, leading to reduced insulin secretion. The positive effects of G LED treatment have potential for metabolic syndrome, diabetes, and pancreatic diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping