PUBLICATION
Cspg4 sculpts oligodendrocyte precursor cell morphology
- Authors
- Bromley-Coolidge, S., Iruegas, D., Appel, B.
- ID
- ZDB-PUB-241121-10
- Date
- 2024
- Source
- Differentiation; research in biological diversity 140: 100819100819 (Journal)
- Registered Authors
- Appel, Bruce
- Keywords
- Extracellular matrix, Glia, Myelination, Neural cell differentiation, Neural cell fate, Zebrafish
- MeSH Terms
-
- Extracellular Matrix/genetics
- Extracellular Matrix/metabolism
- Antigens
- Central Nervous System/cytology
- Central Nervous System/growth & development
- Central Nervous System/metabolism
- Myelin Sheath*/metabolism
- Cell Differentiation*
- Oligodendroglia*/cytology
- Oligodendroglia*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish*/genetics
- Proteoglycans
- Oligodendrocyte Precursor Cells*/cytology
- Oligodendrocyte Precursor Cells*/metabolism
- Animals
- Chondroitin Sulfate Proteoglycans/genetics
- Chondroitin Sulfate Proteoglycans/metabolism
- PubMed
- 39566199 Full text @ Differentiation
Citation
Bromley-Coolidge, S., Iruegas, D., Appel, B. (2024) Cspg4 sculpts oligodendrocyte precursor cell morphology. Differentiation; research in biological diversity. 140:100819100819.
Abstract
The extracellular matrix (ECM) provides critical biochemical and structural cues that regulate neural development. Chondroitin sulfate proteoglycans (CSPGs), a major ECM component, have been implicated in modulating oligodendrocyte precursor cell (OPC) proliferation, migration, and maturation, but their specific roles in oligodendrocyte lineage cell (OLC) development and myelination in vivo remain poorly understood. Here, we use zebrafish as a model system to investigate the spatiotemporal dynamics of ECM deposition and CSPG localization during central nervous system (CNS) development, with a focus on their relationship to OLCs. We demonstrate that ECM components, including CSPGs, are dynamically expressed in distinct spatiotemporal patterns coinciding with OLC development and myelination. We found that zebrafish lacking cspg4 function produced normal numbers of OLCs, which appeared to undergo proper differentiation. However, OPC morphology in mutant larvae was aberrant. Nevertheless, the number and length of myelin sheaths produced by mature oligodendrocytes were unaffected. These data indicate that Cspg4 regulates OPC morphogenesis in vivo, supporting the role of the ECM in neural development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping