PUBLICATION
Tunneling nanotubes enable intercellular transfer in zebrafish embryos
- Authors
- Korenkova, O., Liu, S., Prlesi, I., Pepe, A., Albadri, S., Del Bene, F., Zurzolo, C.
- ID
- ZDB-PUB-241115-7
- Date
- 2024
- Source
- Developmental Cell : (Journal)
- Registered Authors
- Albadri, Shahad, Del Bene, Filippo
- Keywords
- TNT-like structures, TNTs, cytokinetic bridges, cytonemes, intercellular communication, intercellular connections, organelle transfer, tunneling nanotubes, zebrafish embryo, zebrafish gastrula
- MeSH Terms
-
- Biological Transport
- Animals
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Nanotubes*
- Embryo, Nonmammalian*/cytology
- Embryo, Nonmammalian*/metabolism
- Gastrula/metabolism
- Cell Membrane Structures
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Cell Communication*
- PubMed
- 39541978 Full text @ Dev. Cell
Citation
Korenkova, O., Liu, S., Prlesi, I., Pepe, A., Albadri, S., Del Bene, F., Zurzolo, C. (2024) Tunneling nanotubes enable intercellular transfer in zebrafish embryos. Developmental Cell. :.
Abstract
Tunneling nanotubes (TNTs) are thin intercellular connections that facilitate the transport of diverse cargoes, ranging from ions to organelles. While TNT studies have predominantly been conducted in cell cultures, the existence of open-ended TNTs within live organisms remains unverified. Despite the observation of intercellular connections during embryonic development across various species, their functional role in facilitating material transfer between connected cells has not been confirmed. In this study, we performed mosaic labeling of gastrula cells in zebrafish embryos to demonstrate the coexistence of TNT-like structures alongside other cellular protrusions. These embryonic TNT-like connections exhibited a morphology similar to that of TNTs described in cell culture, appeared to have similar formation mechanisms, and could be induced by Eps8 overexpression and CK666 treatment. Most notably, we demonstrated their capability to transfer both soluble cargoes and organelles, thus confirming their open-endedness. This study demonstrates the existence of functional, open-ended TNTs in a living embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping