PUBLICATION
Apigenin attenuates cisplatin-induced hair cell damage in the zebrafish lateral line
- Authors
- Kong, S., Xiao, Y., Chen, L., Jin, Y., Qiao, R., Xu, K., Xu, L., Wang, H.
- ID
- ZDB-PUB-241114-11
- Date
- 2024
- Source
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 194: 115099 (Journal)
- Registered Authors
- Keywords
- apigenin, cisplatin, ototoxicity, therapeutic, zebrafish
- MeSH Terms
-
- Animals
- Antineoplastic Agents/toxicity
- Apoptosis*/drug effects
- Reactive Oxygen Species/metabolism
- Cisplatin*/toxicity
- Ototoxicity/etiology
- Ototoxicity/prevention & control
- Hair Cells, Auditory*/drug effects
- Zebrafish*
- Lateral Line System*/drug effects
- Apigenin*/pharmacology
- Larva/drug effects
- PubMed
- 39521239 Full text @ Food Chem. Toxicol.
Citation
Kong, S., Xiao, Y., Chen, L., Jin, Y., Qiao, R., Xu, K., Xu, L., Wang, H. (2024) Apigenin attenuates cisplatin-induced hair cell damage in the zebrafish lateral line. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 194:115099.
Abstract
Cisplatin, a widely used chemotherapy drug, is notorious for causing ototoxicity, which leads to irreversible sensorineural hearing loss by damaging cochlear sensory hair cells (HCs), spiral ganglion neurons (SGNs), and the stria vascularis (SV). Mechanisms include DNA adduct formation, mitochondrial dysfunction, oxidative stress, and inflammation, ultimately triggering cell death pathways like apoptosis, necroptosis, pyroptosis, or ferroptosis. Apigenin, a natural flavonoid found in various foods and beverages, possesses antioxidant, anti-inflammatory, and anti-tumor properties. Despite these benefits, its potential to mitigate cisplatin-induced ototoxicity remains unexplored. To investigate, we administered varying concentrations of apigenin (1 μM, 20 μM, 100 μM, and 250 μM) alongside cisplatin (200 μM) to zebrafish larvae at 5 days post fertilization. Cisplatin significantly reduced lateral line HCs, impacting auditory function as shown in startle response tests. However, co-administration with apigenin preserved lateral line HCs and mitigated cisplatin-induced hearing loss. In larvae exposed to cisplatin, TUNEL assay confirmed significant HCs apoptosis, which apigenin effectively countered by suppressing reactive oxygen species accumulation in lateral line HCs. RNA-seq analysis highlighted apigenin's role in modulating apoptosis-related pathways, supporting its protective effects against cisplatin-induced ototoxicity. These findings underscore apigenin's potential as a crucial protective agent against cisplatin-induced ototoxicity, meriting further investigation for clinical applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping