PUBLICATION
Fast and light-efficient remote focusing for volumetric voltage imaging
- Authors
- Böhm, U.L., Judkewitz, B.
- ID
- ZDB-PUB-241106-9
- Date
- 2024
- Source
- Nature communications 15: 95559555 (Journal)
- Registered Authors
- Judkewitz, Benjamin
- Keywords
- none
- MeSH Terms
-
- Animals
- Light
- Neurons*/physiology
- Spinal Cord*/diagnostic imaging
- Spinal Cord*/physiology
- Zebrafish*
- PubMed
- 39500891 Full text @ Nat. Commun.
Citation
Böhm, U.L., Judkewitz, B. (2024) Fast and light-efficient remote focusing for volumetric voltage imaging. Nature communications. 15:95559555.
Abstract
Voltage imaging holds great potential for biomedical research by enabling noninvasive recording of the electrical activity of excitable cells such as neurons or cardiomyocytes. Camera-based detection can record from hundreds of cells in parallel, but imaging entire volumes is limited by the need to focus through the sample at high speeds. Remote focusing techniques can remedy this drawback, but have so far been either too slow or light-inefficient. Here, we introduce flipped image remote focusing, a remote focusing method that doubles the light efficiency compared to conventional beamsplitter-based techniques and enables high-speed volumetric voltage imaging at 500 volumes/s. We show the potential of our approach by combining it with light sheet imaging in the zebrafish spinal cord to record from >100 spontaneously active neurons in parallel.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping