PUBLICATION

Fat mass and obesity associated gene and homeobox transcription factor iriquois-3 mRNA profiles in the metabolic tissues of zebrafish are modulated by feeding and food deprivation

Authors
Karimzadeh, K., Uju, C., Zahmatkesh, A., Unniappan, S.
ID
ZDB-PUB-241018-9
Date
2024
Source
General and comparative endocrinology   360: 114621 (Journal)
Registered Authors
Keywords
Appetite, Brain, Food deprivation, Hormones, Nutrition, Periprandial
MeSH Terms
  • Animals
  • Zebrafish*/genetics
  • Zebrafish*/metabolism
  • Homeodomain Proteins*/genetics
  • Homeodomain Proteins*/metabolism
  • Male
  • Female
  • Food Deprivation/physiology
  • Transcription Factors*/genetics
  • Transcription Factors*/metabolism
  • RNA, Messenger*/genetics
  • RNA, Messenger*/metabolism
  • Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics
  • Alpha-Ketoglutarate-Dependent Dioxygenase FTO/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
39414090 Full text @ Gen. Comp. Endocrinol.
Abstract
Fat mass and obesity associated gene (FTO) has been strongly associated with obesity, and it is functionally linked to the homeobox transcription factor iriquois-3 (IRX3). In mammals, FTO and IRX3 are involved in the regulation of food intake and metabolism. This study aimed to determine whether FTO and IRX3are affected by feeding and food unavailability. FTO and IRX3 mRNA and protein were found widely distributed in all tissues examined, including the brain, muscle, gut, and liver. Postprandial increase in the abundance of FTO and IRX3 mRNAs was observed in metabolic tissues of both male and female zebrafish at 1 h post-feeding. Meanwhile, their expression in the brain and gut decreased at 3 h post-feeding, reaching preprandial levels. Additionally, FTO and IRX3 mRNA abundance in examined tissues increased after 7 days of food deprivation, but substantially decreased after refeeding for 24 h. In summary, we report that both FTO and IRX3 are meal-sensitive genes in zebrafish. The fasting-induced increase suggests a possible appetite regulatory role for FTO and IRX3 in zebrafish. These findings highlight the importance of FTO and IRX3 in appetite and metabolic regulation in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping