PUBLICATION
Fat mass and obesity associated gene and homeobox transcription factor iriquois-3 mRNA profiles in the metabolic tissues of zebrafish are modulated by feeding and food deprivation
- Authors
- Karimzadeh, K., Uju, C., Zahmatkesh, A., Unniappan, S.
- ID
- ZDB-PUB-241018-9
- Date
- 2024
- Source
- General and comparative endocrinology 360: 114621 (Journal)
- Registered Authors
- Keywords
- Appetite, Brain, Food deprivation, Hormones, Nutrition, Periprandial
- MeSH Terms
-
- Animals
- RNA, Messenger*/genetics
- RNA, Messenger*/metabolism
- Food Deprivation/physiology
- Homeodomain Proteins*/genetics
- Homeodomain Proteins*/metabolism
- Transcription Factors*/genetics
- Transcription Factors*/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Female
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Alpha-Ketoglutarate-Dependent Dioxygenase FTO/genetics
- Alpha-Ketoglutarate-Dependent Dioxygenase FTO/metabolism
- Male
- PubMed
- 39414090 Full text @ Gen. Comp. Endocrinol.
Citation
Karimzadeh, K., Uju, C., Zahmatkesh, A., Unniappan, S. (2024) Fat mass and obesity associated gene and homeobox transcription factor iriquois-3 mRNA profiles in the metabolic tissues of zebrafish are modulated by feeding and food deprivation. General and comparative endocrinology. 360:114621.
Abstract
Fat mass and obesity associated gene (FTO) has been strongly associated with obesity, and it is functionally linked to the homeobox transcription factor iriquois-3 (IRX3). In mammals, FTO and IRX3 are involved in the regulation of food intake and metabolism. This study aimed to determine whether FTO and IRX3are affected by feeding and food unavailability. FTO and IRX3 mRNA and protein were found widely distributed in all tissues examined, including the brain, muscle, gut, and liver. Postprandial increase in the abundance of FTO and IRX3 mRNAs was observed in metabolic tissues of both male and female zebrafish at 1 h post-feeding. Meanwhile, their expression in the brain and gut decreased at 3 h post-feeding, reaching preprandial levels. Additionally, FTO and IRX3 mRNA abundance in examined tissues increased after 7 days of food deprivation, but substantially decreased after refeeding for 24 h. In summary, we report that both FTO and IRX3 are meal-sensitive genes in zebrafish. The fasting-induced increase suggests a possible appetite regulatory role for FTO and IRX3 in zebrafish. These findings highlight the importance of FTO and IRX3 in appetite and metabolic regulation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping