PUBLICATION
Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals
- Authors
- Guo, K., Kalyviotis, K., Pantazis, P., Rowlands, C.J.
- ID
- ZDB-PUB-241015-8
- Date
- 2024
- Source
- Proceedings of the National Academy of Sciences of the United States of America 121: e2404232121e2404232121 (Journal)
- Registered Authors
- Pantazis, Periklis (Laki)
- Keywords
- Raman spectroscopy, label-free imaging, light sheet microscopy, oblique plane microscopy
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*
- Extracellular Matrix/metabolism
- Heart/diagnostic imaging
- Hyperspectral Imaging/methods
- Microplastics
- Microscopy/methods
- Spectrum Analysis, Raman*/methods
- Zebrafish*/embryology
- PubMed
- 39401353 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Guo, K., Kalyviotis, K., Pantazis, P., Rowlands, C.J. (2024) Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals. Proceedings of the National Academy of Sciences of the United States of America. 121:e2404232121e2404232121.
Abstract
Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named [Formula: see text]-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its function, we use [Formula: see text]-OPM to map and identify microplastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that [Formula: see text]-OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and extracellular matrix composition in the wounded region. Finally, we use [Formula: see text]-OPM to synchronize and average 36,800 individual frames to obtain hyperspectral videos of a zebrafish embryo's beating heart at an effective 28 frames per second, recording compositional changes throughout the cardiac cycle.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping