PUBLICATION
Plk4 regulates centriole duplication in the embryonic development of zebrafish
- Authors
- Mu, Z., Zheng, P., Liu, S., Kang, Y., Xie, H.
- ID
- ZDB-PUB-240922-6
- Date
- 2024
- Source
- Developmental Biology 517: 148-156 (Journal)
- Registered Authors
- Keywords
- Centriole duplication, Embryonic development, Plk4, Zebrafish
- MeSH Terms
-
- Animals
- Cell Division
- Centrosome/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- Zebrafish*/embryology
- Zebrafish*/metabolism
- Embryonic Development*/genetics
- Centrioles*/metabolism
- Protein Domains
- Embryo, Nonmammalian/metabolism
- Mutation/genetics
- Cilia/metabolism
- Gene Expression Regulation, Developmental
- Protein Serine-Threonine Kinases*/genetics
- Protein Serine-Threonine Kinases*/metabolism
- Apoptosis/genetics
- PubMed
- 39304174 Full text @ Dev. Biol.
Citation
Mu, Z., Zheng, P., Liu, S., Kang, Y., Xie, H. (2024) Plk4 regulates centriole duplication in the embryonic development of zebrafish. Developmental Biology. 517:148-156.
Abstract
PLK4 plays a crucial role in centriole duplication, which is essential for maintaining cellular processes such as cell division, cytoskeletal stability, and cilia formation. However, the mechanisms of PLK4 remain incompletely understood, especially in the embryonic development of vertebrate species. In this study, we observed that Plk4 dysfunction led to abnormal embryonic development in zebrafish, characterized by symptoms such as dark and wrinkled skin, microphthalmia, and body axis curvature. In plk4 mutants, defects in centriole duplication led to abnormal cell division, apoptosis, and ciliogenesis defects. Moreover, overexpression of plk4 in zebrafish embryos caused excessive centrosome amplification, disrupting embryonic gastrulation through abnormal cell division and ultimately resulting in embryonic lethality. Furthermore, we identified the "cryptic" polo box (CPB) domain, consisting of two PBs (PB1 and PB2), as the critical centrosome localization domain of Plk4. Surprisingly, overexpression of these two PB domains alone was sufficient to induce embryonic lethality. Additionally, we discovered a truncated form of CPB that localizes to the centrosome without causing defects in embryonic development. Our results demonstrate that Plk4 tightly controls centriole duplication, which is essential for early embryonic development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping