PUBLICATION
Tumour vasculature at single-cell resolution
- Authors
- Pan, X., Li, X., Dong, L., Liu, T., Zhang, M., Zhang, L., Zhang, X., Huang, L., Shi, W., Sun, H., Fang, Z., Sun, J., Huang, Y., Shao, H., Wang, Y., Yin, M.
- ID
- ZDB-PUB-240809-1
- Date
- 2024
- Source
- Nature 632: 429436429-436 (Journal)
- Registered Authors
- Wang, Yeqi
- Keywords
- none
- MeSH Terms
-
- Animals
- Neoplasms*/blood supply
- Neoplasms*/classification
- Neoplasms*/drug therapy
- Neoplasms*/pathology
- Signal Transduction
- Zebrafish
- Pericytes/cytology
- Pericytes/metabolism
- Pericytes/pathology
- Cell Differentiation
- Prognosis
- Lymphangiogenesis
- Tumor Microenvironment
- Vascular Endothelial Growth Factor A/antagonists & inhibitors
- Endothelial Cells*/cytology
- Endothelial Cells*/immunology
- Endothelial Cells*/metabolism
- Receptors, Notch/metabolism
- Single-Cell Analysis*
- Endoplasmic Reticulum Stress
- Neovascularization, Pathologic*/pathology
- Cell Lineage
- Humans
- Disease Progression
- Antigen Presentation
- Cell Communication
- PubMed
- 38987599 Full text @ Nature
Citation
Pan, X., Li, X., Dong, L., Liu, T., Zhang, M., Zhang, L., Zhang, X., Huang, L., Shi, W., Sun, H., Fang, Z., Sun, J., Huang, Y., Shao, H., Wang, Y., Yin, M. (2024) Tumour vasculature at single-cell resolution. Nature. 632:429436429-436.
Abstract
Tumours can obtain nutrients and oxygen required to progress and metastasize through the blood supply1. Inducing angiogenesis involves the sprouting of established vessel beds and their maturation into an organized network2,3. Here we generate a comprehensive atlas of tumour vasculature at single-cell resolution, encompassing approximately 200,000 cells from 372 donors representing 31 cancer types. Trajectory inference suggested that tumour angiogenesis was initiated from venous endothelial cells and extended towards arterial endothelial cells. As neovascularization elongates (through angiogenic stages SI, SII and SIII), APLN+ tip cells at the SI stage (APLN+ TipSI) advanced to TipSIII cells with increased Notch signalling. Meanwhile, stalk cells, following tip cells, transitioned from high chemokine expression to elevated TEK (also known as Tie2) expression. Moreover, APLN+ TipSI cells not only were associated with disease progression and poor prognosis but also hold promise for predicting response to anti-VEGF therapy. Lymphatic endothelial cells demonstrated two distinct differentiation lineages: one responsible for lymphangiogenesis and the other involved in antigen presentation. In pericytes, endoplasmic reticulum stress was associated with the proangiogenic BASP1+ matrix-producing pericytes. Furthermore, intercellular communication analysis showed that neovascular endothelial cells could shape an immunosuppressive microenvironment conducive to angiogenesis. This study depicts the complexity of tumour vasculature and has potential clinical significance for anti-angiogenic therapy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping