PUBLICATION
Construction of an effective near-infrared fluorescence "turn-on" probe for hydrogen sulfide detection and imaging in living inflammatory cell and zebrafish models
- Authors
- Chen, S., Liu, T., Yuan, X., Zhou, L.
- ID
- ZDB-PUB-240729-15
- Date
- 2024
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 323: 124863124863 (Journal)
- Registered Authors
- Keywords
- Cell imaging, Fluorescence probe, Hydrogen sulfide, Near-infrared imaging, Zebrafish
- MeSH Terms
-
- Spectrometry, Fluorescence/methods
- Humans
- Spectroscopy, Near-Infrared/methods
- Inflammation*
- Fluorescent Dyes*/chemistry
- Mice
- RAW 264.7 Cells
- Animals
- Limit of Detection
- Zebrafish*
- Optical Imaging/methods
- Hydrogen Sulfide*/analysis
- PubMed
- 39068845 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Chen, S., Liu, T., Yuan, X., Zhou, L. (2024) Construction of an effective near-infrared fluorescence "turn-on" probe for hydrogen sulfide detection and imaging in living inflammatory cell and zebrafish models. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 323:124863124863.
Abstract
Hydrogen sulfide (H2S) can act as a gaseous signaling mediator closely associated with inflammation development. In this work, we designed a fluorescence turn-on near-infrared (NIR) fluorescent probe CIT-H2S based on Intermolecular Charge Transfer (ICT) for the detection of H2S in living inflammatory cells and zebrafish. On this basis, a dicyanoisophorone fluorophore was chosen as the fluorescence signal reporting group of CIT-H2S, and an azide group was constructed as the recognition group of H2S. CIT-H2S is characterized by high selectivity and sensitivity for H2S over other interference species. The fluorescence intensity at 661 nm showed good linearity in the range of H2S concentration from 0 to 10 μM, with an excellent limit of detection (LOD) as low as 81.52 nM. Impressively, CIT-H2S has been visualized for detecting H2S in drug-induced inflammatory cell and zebrafish models, thus indicating that CIT-H2S is a robust tool with the ability to study the occurrence and development of hydrogen sulfide and inflammation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping