PUBLICATION
Dzip1 is dynamically expressed in the vertebrate germline and regulates the development of Xenopus primordial germ cells
- Authors
- Turgeon, A., Fu, J., Divyanshi, ., Ma, M., Jin, Z., Hwang, H., Li, M., Qiao, H., Mei, W., Yang, J.
- ID
- ZDB-PUB-240617-7
- Date
- 2024
- Source
- Developmental Biology 514: 28-36 (Journal)
- Registered Authors
- Keywords
- Dazl, Dzip1, Xenopus, germ plasm, germline development, primordial germ cell
- MeSH Terms
-
- Oogenesis/genetics
- Animals
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Gene Expression Regulation, Developmental*
- Female
- RNA-Binding Proteins*/genetics
- RNA-Binding Proteins*/metabolism
- Adaptor Proteins, Signal Transducing*/metabolism
- Xenopus laevis*/embryology
- Xenopus laevis*/genetics
- Xenopus laevis*/metabolism
- Xenopus Proteins*/genetics
- Xenopus Proteins*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Germ Cells*/cytology
- Germ Cells*/metabolism
- PubMed
- 38880277 Full text @ Dev. Biol.
Citation
Turgeon, A., Fu, J., Divyanshi, ., Ma, M., Jin, Z., Hwang, H., Li, M., Qiao, H., Mei, W., Yang, J. (2024) Dzip1 is dynamically expressed in the vertebrate germline and regulates the development of Xenopus primordial germ cells. Developmental Biology. 514:28-36.
Abstract
Primordial germ cells (PGCs) are the precursors of sperms and oocytes. Proper development of PGCs is crucial for the survival of the species. In many organisms, factors responsible for PGC development are synthesized during early oogenesis and assembled into the germ plasm. During early embryonic development, germ plasm is inherited by a few cells, leading to the formation of PGCs. While germline development has been extensively studied, how components of the germ plasm regulate PGC development is not fully understood. Here, we report that Dzip1 is dynamically expressed in vertebrate germline and is a novel component of the germ plasm in Xenopus and zebrafish. Knockdown of Dzip1 impairs PGC development in Xenopus embryos. At the molecular level, Dzip1 physically interacts with Dazl, an evolutionarily conserved RNA-binding protein that plays a multifaced role during germline development. We further showed that the sequence between amino acid residues 282 and 550 of Dzip1 is responsible for binding to Dazl. Disruption of the binding between Dzip1 and Dazl leads to defective PGC development. Taken together, our results presented here demonstrate that Dzip1 is dynamically expressed in the vertebrate germline and plays a novel function during Xenopus PGC development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping