PUBLICATION
Developmental toxicity and mechanism of dibutyl phthalate and alternative diisobutyl phthalate in the early life stages of zebrafish (Danio rerio)
- Authors
- Tao, H.Y., Shi, J., Zhang, J., Ge, H., Zhang, M., Li, X.Y.
- ID
- ZDB-PUB-240527-8
- Date
- 2024
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 272: 106962106962 (Journal)
- Registered Authors
- Keywords
- Developmental toxicity, Dibutyl phthalate, Diisobutyl phthalate, Gene transcription, Joint toxicity, Thyroid hormone
- MeSH Terms
-
- Dibutyl Phthalate*/analogs & derivatives
- Dibutyl Phthalate*/toxicity
- Embryo, Nonmammalian*/drug effects
- Larva/drug effects
- Larva/genetics
- Larva/growth & development
- Gene Expression Regulation, Developmental/drug effects
- Phthalic Acids/toxicity
- Thyroid Gland/drug effects
- Zebrafish*/genetics
- Animals
- Water Pollutants, Chemical*/toxicity
- Thyroid Hormones/metabolism
- PubMed
- 38797068 Full text @ Aquat. Toxicol.
Citation
Tao, H.Y., Shi, J., Zhang, J., Ge, H., Zhang, M., Li, X.Y. (2024) Developmental toxicity and mechanism of dibutyl phthalate and alternative diisobutyl phthalate in the early life stages of zebrafish (Danio rerio). Aquatic toxicology (Amsterdam, Netherlands). 272:106962106962.
Abstract
Diisobutyl phthalate (DiBP), is widely chemical replacement for Dibutyl phthalate (DBP). Although DBP and DiBP have been detected in surface water worldwide, few studies to date have systematically assessed the risks of DBP and its alternatives to aquatic organisms. The present study compared DBP and DiBP for their individual and joint toxicity as well as thyroid hormone levels in zebrafish embryo. Transcripts of key genes related to the hypothalamic-pituitary-thyroid (HPT) axis were investigated in developing zebrafish larvae by application of real time polymerase chain reaction. The median half-lethal concentrations of DBP and DiBP to zebrafish at 96 h were 0.545 mg L-1 and 1.149 mg L-1, respectively. The joint toxic effect of DBP-DiBP (0.25-0.53 mg L-1) with the same ratio showed a synergistic effect. Thyroid hormones levels increased with exposure to 10 μg L-1 of DBP or 50 μg L-1 of DiBP, and exposure to both compounds significantly increased thyroid gland-specific transcription of thyroglobulin gene (tg), hyronine deiodinase (dio2), and transthyretin (ttr), indicating an adverse effect associated with the HPT axis. Molecular docking results indicated that DBP (-7.10 kcal/M and -7.53 kcal/M) and DiBP (-6.63 kcal/M and -7.42 kcal/M) had the same docking energy with thyroid hormone receptors. Our data facilities an understand of potential harmful effects of DBP and its alternative (DiBP).
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping