PUBLICATION
Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle
- Authors
- Zhang, R.J., Vermot, J., Gherardi, R., Fukui, H., Chow, R.W.
- ID
- ZDB-PUB-240527-13
- Date
- 2024
- Source
- Bio-protocol 14: e4989e4989 (Journal)
- Registered Authors
- Chow, Renee
- Keywords
- Cardiac cycle, Heart valve development, Image analysis, Phase matching, Software development, Zebrafish embryos
- MeSH Terms
- none
- PubMed
- 38798980 Full text @ Bio Protoc
Citation
Zhang, R.J., Vermot, J., Gherardi, R., Fukui, H., Chow, R.W. (2024) Calcium Signal Analysis in the Zebrafish Heart via Phase Matching of the Cardiac Cycle. Bio-protocol. 14:e4989e4989.
Abstract
Calcium signalling in the endocardium is critical for heart valve development. Calcium ion pulses in the endocardium are generated in response to mechanical forces due to blood flow and can be visualised in the beating zebrafish heart using a genetically encoded calcium indicator such as GCaMP7a. Analysing these pulses is challenging because of the rapid movement of the heart during heartbeat. This protocol outlines an imaging analysis method used to phase-match the cardiac cycle in single z-slice movies of the beating heart, allowing easy measurement of the calcium signal. Key features • Software to synchronise and analyse frames from movies of the beating heart corresponding to a user-defined phase of the cardiac cycle. • Software to measure the fluorescence intensity of the beating heart corresponding to a user-defined region of interest.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping