PUBLICATION
Microbial Polysaccharides Extracted from Different Mature Muds of the Euganean Thermal District Show Similar Anti-Inflammatory Activity In Vivo
- Authors
- Caichiolo, M., Zampieri, R.M., Adessi, A., Ciani, M., Caldara, F., Dalla Valle, L., La Rocca, N.
- ID
- ZDB-PUB-240512-13
- Date
- 2024
- Source
- International Journal of Molecular Sciences 25(9): (Journal)
- Registered Authors
- Dalla Valle, Luisa, Zampieri, Raffaella Margherita
- Keywords
- bioactive molecule, cyanobacteria, inflammation, microbiota, peloid, polysaccharide, thermal mud, zebrafish
- MeSH Terms
-
- Polysaccharides, Bacterial/chemistry
- Polysaccharides, Bacterial/pharmacology
- Mud Therapy
- Zebrafish*
- Animals
- Anti-Inflammatory Agents*/chemistry
- Anti-Inflammatory Agents*/pharmacology
- Italy
- Microbiota/drug effects
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification
- Polysaccharides/pharmacology
- PubMed
- 38732217 Full text @ Int. J. Mol. Sci.
Citation
Caichiolo, M., Zampieri, R.M., Adessi, A., Ciani, M., Caldara, F., Dalla Valle, L., La Rocca, N. (2024) Microbial Polysaccharides Extracted from Different Mature Muds of the Euganean Thermal District Show Similar Anti-Inflammatory Activity In Vivo. International Journal of Molecular Sciences. 25(9):.
Abstract
The Euganean Thermal District, situated in North-East Italy, is one of Europe's largest and oldest thermal centres. The topical application of its therapeutic thermal muds is recognised by the Italian Health System as a beneficial treatment for patients suffering from arthro-rheumatic diseases. Polysaccharides produced by the mud microbiota have been recently identified as anti-inflammatory bioactive molecules. In this paper we analysed the efficacy of Microbial-Polysaccharides (M-PS) derived from mature muds obtained at different maturation temperatures, both within and outside the codified traditional mud maturation range. M-PSs were extracted from six mature muds produced by five spas of the Euganean Thermal District and investigated for their chemical properties, monosaccharide composition and in vivo anti-inflammatory potential, using the zebrafish model organism. Additionally, mature muds were characterized for their microbiota composition using Next-Generation Sequencing. The results showed that all M-PSs exhibit similar anti-inflammatory potential, referable to their comparable chemical composition. This consistency was observed despite changes in cyanobacteria populations, suggesting a possible role of the entire microbial community in shaping the properties of these biomolecules. These findings highlight the importance of scientific research in untangling the origins of the therapeutic efficacy of Euganean Thermal muds in the treatment of chronic inflammatory conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping